PDBsum entry 2pxh

Ligase

PDB id

2pxh

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Contents

			Protein chain

					306 a.a. *

			Ligands

					BP5

			Waters ×278

* Residue conservation analysis

PDB id:

2pxh

Links

Name:

Ligase

Title:

Crystal structure of a bipyridylalanyl-tRNA synthetase

Structure:

Tyrosyl-tRNA synthetase. Chain: a. Synonym: tyrosine--tRNA ligase, tyrrs. Engineered: yes

Source:

Methanocaldococcus jannaschii. Organism_taxid: 2190. Gene: tyrosyl-tRNA synthetase. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

1.97Å

R-factor:

0.193

R-free:

0.242

Authors:

W.Liu,J.Xie,P.G.Schultz

Key ref:

J.Xie et al. (2007). A genetically encoded bidentate, metal-binding amino acid. Angew Chem Int Ed Engl, 46, 9239-9242. PubMed id: 17893898

Date:

14-May-07

Release date:

05-Feb-08

PROCHECK

	Headers

	References

Protein chain

Q57834 (SYY_METJA) - Tyrosine--tRNA ligase from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)

Seq: Struc:					306 a.a. 306 a.a.*

Key:

PfamA domain

Secondary structure

CATH domain

* PDB and UniProt seqs differ at 7 residue positions (black crosses)



		Enzyme reactions

Enzyme class:

E.C.6.1.1.1 - tyrosine--tRNA ligase.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Reaction:

tRNA(Tyr) + L-tyrosine + ATP = L-tyrosyl-tRNA(Tyr) + AMP + diphosphate + H⁺

tRNA(Tyr)

L-tyrosine
Bound ligand (Het Group name = BP5)
matches with 63.16% similarity

ATP

L-tyrosyl-tRNA(Tyr)

AMP

diphosphate

H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

	Angew Chem Int Ed Engl 46:9239-9242 (2007)
PubMed id: 17893898

A genetically encoded bidentate, metal-binding amino acid.
J.Xie, W.Liu, P.G.Schultz.

ABSTRACT

No abstract given.

Literature references that cite this PDB file's key reference

PubMed id

Reference

20179771

F.Wang, S.Robbins, J.Guo, W.Shen, and P.G.Schultz (2010).
Genetic incorporation of unnatural amino acids into proteins in Mycobacterium tuberculosis.

PLoS One, 5, e9354.

19318213

Q.Wang, A.R.Parrish, and L.Wang (2009).
Expanding the genetic code for biological studies.

Chem Biol, 16, 323-336.

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time.