PDBsum entry 2f8x

Transcription/DNA

PDB id: 2f8x

Contents

Protein chains

424 a.a.

218 a.a.

55 a.a.

DNA/RNA

Waters ×10

References listed in PDB file

Key reference

Title

Structural basis for cooperativity in recruitment of maml coactivators to notch transcription complexes.

Authors

Y.Nam, P.Sliz, L.Song, J.C.Aster, S.C.Blacklow.

Ref.

Cell, 2006, 124, 973-983. [DOI no: 10.1016/j.cell.2005.12.037]

PubMed id

16530044

Abstract

Notch receptors transduce essential developmental signals between neighboring cells by forming a complex that leads to transcription of target genes upon activation. We report here the crystal structure of a Notch transcriptional activation complex containing the ankyrin domain of human Notch1 (ANK), the transcription factor CSL on cognate DNA, and a polypeptide from the coactivator Mastermind-like-1 (MAML-1). Together, CSL and ANK create a groove to bind the MAML-1 polypeptide as a kinked, 70 A helix. The composite binding surface likely restricts the recruitment of MAML proteins to promoters on which Notch:CSL complexes have been preassembled, ensuring tight transcriptional control of Notch target genes.

Figure 3.
Figure 3. Interactions between ANK and RHR-C

Figure 4.
Figure 4. Cooperative Binding of MAML-1 by ANK and CSL

The above figures are reprinted by permission from Cell Press: Cell (2006, 124, 973-983) copyright 2006.