PDBsum entry 2xrm

Hydrolase

PDB id: 2xrm

Contents

Protein chain

301 a.a.

Ligands

1PE

Metals

_NA

_SR

_CA

Waters ×26

PDB id:

2xrm

Name:

Hydrolase

Title:

Processed intracellular subtilisin from b. Clausii

Structure:

Intracellular subtilisin protease. Chain: a. Fragment: processed protein, residues 19-321. Engineered: yes. Mutation: yes. Other_details: n terminal 18 residues deleted

Source:

Bacillus clausii. Organism_taxid: 79880. Expressed in: escherichia coli bl21. Expression_system_taxid: 511693. Other_details: isolated from a novozymes strain b. Clausii strain - strain number available on request.

Resolution:

2.60Å R-factor: 0.202 R-free: 0.281

Authors:

M.Gamble,G.Kunze,E.J.Dodson,D.D.Jones,K.S.Wilson

Key ref:

M.Gamble et al. (2011). Regulation of an intracellular subtilisin protease activity by a short propeptide sequence through an original combined dual mechanism. Proc Natl Acad Sci U S A, 108, 3536-3541. PubMed id: 21307308

Date:

20-Sep-10 Release date: 16-Mar-11

Protein chain

D0AB41  (D0AB41_ALKCL) -  Intracellular subtilisin protease from Alkalihalobacillus clausii

Seq: 321 a.a.

Struc: 301 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.3.4.21.62 - subtilisin.
• Reaction: Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyzes peptide amides.

Proc Natl Acad Sci U S A 108:3536-3541 (2011)

PubMed id: 21307308

Regulation of an intracellular subtilisin protease activity by a short propeptide sequence through an original combined dual mechanism.

M.Gamble, G.Künze, E.J.Dodson, K.S.Wilson, D.D.Jones.

ABSTRACT

No abstract given.