PDBsum entry 2liz

Hydrolase

PDB id: 2liz

Contents

Protein chain

120 a.a.

PDB id:

2liz

Name:

Hydrolase

Title:

Nmr solution structure of c-terminal domain of sars-cov main protease in 2.5m urea

Structure:

3c-like proteinase. Chain: a. Fragment: unp residues 3427-3546. Synonym: 3cl-pro, 3clp, nsp5. Engineered: yes

Source:

Sars coronavirus. Sars-cov. Organism_taxid: 227859. Gene: 1a. Expressed in: escherichia coli. Expression_system_taxid: 562.

NMR struc:

20 models

Authors:

B.Xia,X.Kang

Key ref:

B.Xia and x.kang Nmr solution structure of c-Terminal domain of sars-C protease in 2.5m urea. To be published, . PubMed id: 22927388

Date:

02-Sep-11 Release date: 05-Sep-12

Protein chain

P0C6U8  (R1A_CVHSA) -  Replicase polyprotein 1a from Severe acute respiratory syndrome coronavirus

Seq: 4382 a.a.

Struc: 120 a.a.

Enzyme reactions

• Enzyme class 1: E.C.2.7.7.50 - mRNA guanylyltransferase.
• Reaction: a 5'-end diphospho-ribonucleoside in mRNA + GTP + H⁺ = a 5'-end (5'-triphosphoguanosine)-ribonucleoside in mRNA + diphosphate
• Enzyme class 2: E.C.3.4.19.12 - ubiquitinyl hydrolase 1.
• Reaction: Thiol-dependent hydrolysis of ester, thiolester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
• Enzyme class 3: E.C.3.4.22.- - ?????
• Enzyme class 4: E.C.3.4.22.69 - Sars coronavirus main proteinase.

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site