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PDBsum entry 1awc
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protein dna_rna Protein-protein interface(s) links
Transcription/DNA PDB id
1awc

 

 

 

 

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Contents
Protein chains
110 a.a. *
153 a.a. *
DNA/RNA
Waters ×46
* Residue conservation analysis
PDB id:
1awc
Name: Transcription/DNA
Title: Mouse gabp alpha/beta domain bound to DNA
Structure: DNA (5'-d( Ap Ap (Bru) p Gp Ap Cp Cp Gp Gp Ap Ap Gp Tp Ap (Cbr)p Ap Cp (Cbr)p Gp Gp A)-3'). Chain: d. Engineered: yes. DNA (5'-d( Tp Tp Cp Cp Gp Gp (Bru)p Gp (Bru) p Ap Cp Tp Tp Cp Cp Gp Gp Tp Cp Ap T)-3'). Chain: e. Engineered: yes. Protein (ga binding protein alpha).
Source: Synthetic: yes. Mus musculus. House mouse. Organism_taxid: 10090. Gene: gabp alpha. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Gene: gabp beta 1.
Biol. unit: Tetramer (from PQS)
Resolution:
2.15Å     R-factor:   0.211     R-free:   0.282
Authors: A.H.Batchelor,C.Wolberger
Key ref:
A.H.Batchelor et al. (1998). The structure of GABPalpha/beta: an ETS domain- ankyrin repeat heterodimer bound to DNA. Science, 279, 1037-1041. PubMed id: 9461436 DOI: 10.1126/science.279.5353.1037
Date:
01-Oct-97     Release date:   18-Mar-98    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
Q00422  (GABPA_MOUSE) -  GA-binding protein alpha chain from Mus musculus
Seq:
Struc: 		454 a.a.
110 a.a.
Protein chain
Pfam   ArchSchema ?
Q00420  (GABP1_MOUSE) -  GA-binding protein subunit beta-1 from Mus musculus
Seq:
Struc: 		383 a.a.
153 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

DNA/RNA chains
  A-A-BRU-G-A-C-C-G-G-A-A-G-T-A-CBR-A-C-CBR-G-G-A 21 bases
  T-T-C-C-G-G-BRU-G-BRU-A-C-T-T-C-C-G-G-T-C-A-T 21 bases

 

 
DOI no: 10.1126/science.279.5353.1037 Science 279:1037-1041 (1998)
PubMed id: 9461436  
 
 
The structure of GABPalpha/beta: an ETS domain- ankyrin repeat heterodimer bound to DNA.
A.H.Batchelor, D.E.Piper, F.C.de la Brousse, S.L.McKnight, C.Wolberger.
 
  ABSTRACT  
 
GA-binding protein (GABP) is a transcriptional regulator composed of two structurally dissimilar subunits. The alpha subunit contains a DNA-binding domain that is a member of the ETS family, whereas the beta subunit contains a series of ankyrin repeats. The crystal structure of a ternary complex containing a GABPalpha/beta ETS domain-ankyrin repeat heterodimer bound to DNA was determined at 2. 15 angstrom resolution. The structure shows how an ETS domain protein can recruit a partner protein using both the ETS domain and a carboxyl-terminal extension and provides a view of an extensive protein-protein interface formed by a set of ankyrin repeats. The structure also reveals how the GABPalpha ETS domain binds to its core GGA DNA-recognition motif.
 
  Selected figure(s)  
 
Figure 4.
Fig. 4. GABP -DNA interactions. (A) GABP -DNA interactions in the major groove. Only bases making hydrogen bond contacts with GABP are shown. (B) Interactions between the recognition helix of PU.1 and the major groove (7). (C) Indirect GABP -DNA interaction mediated by Lys69 of GABP and Gln321 of GABP . 		Figure 5.
Fig. 5. Comparison of the GABP crystal structure and Ets-1 NMR structure (9). Each structure is positioned with its ETS domain in the same orientation.
 
  The above figures are reprinted by permission from the AAAs: Science (1998, 279, 1037-1041) copyright 1998.  
  Figures were selected by an automated process.  

Literature references that cite this PDB file's key reference

  PubMed id Reference
21333652 B.Busby, T.Oashi, C.D.Willis, M.A.Ackermann, A.Kontrogianni-Konstantopoulos, A.D.Mackerell, and R.J.Bloch (2011).
Electrostatic interactions mediate binding of obscurin to small ankyrin 1: biochemical and molecular modeling studies.
  J Mol Biol, 408, 321-334.  
21458342 J.Karanicolas, J.E.Corn, I.Chen, L.A.Joachimiak, O.Dym, S.H.Peck, S.Albeck, T.Unger, W.Hu, G.Liu, S.Delbecq, G.T.Montelione, C.P.Spiegel, D.R.Liu, and D.Baker (2011).
A de novo protein binding pair by computational design and directed evolution.
  Mol Cell, 42, 250-260.
PDB codes: 3q9n 3q9u 3qa9
20028808 F.Habyarimana, C.T.Price, M.Santic, S.Al-Khodor, and Y.A.Kwaik (2010).
Molecular characterization of the Dot/Icm-translocated AnkH and AnkJ eukaryotic-like effectors of Legionella pneumophila.
  Infect Immun, 78, 1123-1134.  
20517297 G.H.Wei, G.Badis, M.F.Berger, T.Kivioja, K.Palin, M.Enge, M.Bonke, A.Jolma, M.Varjosalo, A.R.Gehrke, J.Yan, S.Talukder, M.Turunen, M.Taipale, H.G.Stunnenberg, E.Ukkonen, T.R.Hughes, M.L.Bulyk, and J.Taipale (2010).
Genome-wide analysis of ETS-family DNA-binding in vitro and in vivo.
  EMBO J, 29, 2147-2160.  
  20517466 M.Uittenbogaard, K.K.Baxter, and A.Chiaramello (2010).
The neurogenic basic helix-loop-helix transcription factor NeuroD6 confers tolerance to oxidative stress by triggering an antioxidant response and sustaining the mitochondrial biomass.
  ASN Neuro, 2, e00034.  
21053367 N.Fahham, S.Sardari, S.N.Ostad, B.Vaziri, and M.H.Ghahremani (2010).
C-terminal domain of p16(INK4a) is adequate in inducing cell cycle arrest, growth inhibition and CDK4/6 interaction similar to the full length protein in HT-1080 fibrosarcoma cells.
  J Cell Biochem, 111, 1598-1606.  
20522024 T.Shimokawa, S.Nunomura, Y.Enomoto, and C.Ra (2010).
Amino acid residues in the beta3 strand and subsequent loop of the conserved ETS domain that mediate basic leucine zipper (bZIP) recruitment and potentially distinguish functional attributes of Ets proteins.
  Biochem J, 430, 129-139.  
20079749 V.B.Agarkar, N.D.Babayeva, P.J.Wilder, A.Rizzino, and T.H.Tahirov (2010).
Crystal structure of mouse Elf3 C-terminal DNA-binding domain in complex with type II TGF-beta receptor promoter DNA.
  J Mol Biol, 397, 278-289.
PDB code: 3jtg
19129477 A.Sandqvist, J.K.Björk, M.Akerfelt, Z.Chitikova, A.Grichine, C.Vourc'h, C.Jolly, T.A.Salminen, Y.Nymalm, and L.Sistonen (2009).
Heterotrimerization of heat-shock factors 1 and 2 provides a transcriptional switch in response to distinct stimuli.
  Mol Biol Cell, 20, 1340-1347.  
19742324 C.Baltzer, S.K.Tiefenböck, M.Marti, and C.Frei (2009).
Nutrition controls mitochondrial biogenesis in the Drosophila adipose tissue through Delg and cyclin D/Cdk4.
  PLoS One, 4, e6935.  
19616560 D.Fitzsimmons, K.Lukin, R.Lutz, C.W.Garvie, C.Wolberger, and J.Hagman (2009).
Highly cooperative recruitment of Ets-1 and release of autoinhibition by Pax5.
  J Mol Biol, 392, 452-464.  
19217849 H.Kamberaj, and A.van der Vaart (2009).
Correlated motions and interactions at the onset of the DNA-induced partial unfolding of Ets-1.
  Biophys J, 96, 1307-1317.  
19421232 K.C.Yoon, J.A.Bae, H.J.Park, S.K.Im, H.J.Oh, X.H.Lin, M.Y.Kim, J.H.Lee, S.E.Lee, K.Y.Ahn, and K.K.Kim (2009).
Subconjunctival gene delivery of the transcription factor GA-binding protein delays corneal neovascularization in a mouse model.
  Gene Ther, 16, 973-981.  
19305498 N.Guillon, F.Tirode, V.Boeva, A.Zynovyev, E.Barillot, and O.Delattre (2009).
The oncogenic EWS-FLI1 protein binds in vivo GGAA microsatellite sequences with potential transcriptional activation function.
  PLoS ONE, 4, e4932.  
19776120 S.J.Werden, J.Lanchbury, D.Shattuck, C.Neff, M.Dufford, and G.McFadden (2009).
The myxoma virus m-t5 ankyrin repeat host range protein is a novel adaptor that coordinately links the cellular signaling pathways mediated by Akt and Skp1 in virus-infected cells.
  J Virol, 83, 12068-12083.  
19530232 W.A.Witkowski, and J.A.Hardy (2009).
L2' loop is critical for caspase-7 active site formation.
  Protein Sci, 18, 1459-1468.
PDB code: 3h1p
19117955 Y.Yang, X.Wang, C.A.Hawkins, K.Chen, J.Vaynberg, X.Mao, Y.Tu, X.Zuo, J.Wang, Y.X.Wang, C.Wu, N.Tjandra, and J.Qin (2009).
Structural basis of focal adhesion localization of LIM-only adaptor PINCH by integrin-linked kinase.
  J Biol Chem, 284, 5836-5844.  
18480049 D.Fox, I.Le Trong, P.Rajagopal, P.S.Brzovic, R.E.Stenkamp, and R.E.Klevit (2008).
Crystal structure of the BARD1 ankyrin repeat domain and its functional consequences.
  J Biol Chem, 283, 21179-21186.
PDB code: 3c5r
18566588 E.P.Lamber, L.Vanhille, L.C.Textor, G.S.Kachalova, M.H.Sieweke, and M.Wilmanns (2008).
Regulation of the transcription factor Ets-1 by DNA-mediated homo-dimerization.
  EMBO J, 27, 2006-2017.
PDB code: 2nny
18279343 F.Habyarimana, S.Al-Khodor, A.Kalia, J.E.Graham, C.T.Price, M.T.Garcia, and Y.A.Kwaik (2008).
Role for the Ankyrin eukaryotic-like genes of Legionella pneumophila in parasitism of protozoan hosts and human macrophages.
  Environ Microbiol, 10, 1460-1474.  
18295234 H.S.Kang, M.L.Nelson, C.D.Mackereth, M.Schärpf, B.J.Graves, and L.P.McIntosh (2008).
Identification and structural characterization of a CBP/p300-binding domain from the ETS family transcription factor GABP alpha.
  J Mol Biol, 377, 636-646.
PDB code: 2juo
  18811729 S.Al-Khodor, C.T.Price, F.Habyarimana, A.Kalia, and Y.Abu Kwaik (2008).
A Dot/Icm-translocated ankyrin protein of Legionella pneumophila is required for intracellular proliferation within human macrophages and protozoa.
  Mol Microbiol, 70, 908-923.  
17485447 A.Jaworski, C.L.Smith, and S.J.Burden (2007).
GA-binding protein is dispensable for neuromuscular synapse formation and synapse-specific gene expression.
  Mol Cell Biol, 27, 5040-5046.  
17171436 F.Wang, J.Hu, P.Song, and W.Gong (2007).
Two novel transcripts encoding two Ankyrin repeat containing proteins have preponderant expression during the mouse spermatogenesis.
  Mol Biol Rep, 34, 249-260.  
17652178 P.C.Hollenhorst, A.A.Shah, C.Hopkins, and B.J.Graves (2007).
Genome-wide analyses reveal properties of redundant and specific promoter occupancy within the ETS gene family.
  Genes Dev, 21, 1882-1894.  
17334656 T.Chiba, J.Yao, Y.Higami, I.Shimokawa, M.Hosokawa, and K.Higuchi (2007).
Identification of differentially expressed genes in senescence-accelerated mouse testes by suppression subtractive hybridization analysis.
  Mamm Genome, 18, 105-112.  
16493627 H.K.Binz, A.Kohl, A.Plückthun, and M.G.Grütter (2006).
Crystal structure of a consensus-designed ankyrin repeat protein: implications for stability.
  Proteins, 65, 280-284.
PDB code: 2bkg
16911870 H.P.Kim, J.Imbert, and W.J.Leonard (2006).
Both integrated and differential regulation of components of the IL-2/IL-2 receptor system.
  Cytokine Growth Factor Rev, 17, 349-366.  
16948156 H.Yu, A.Kohl, H.K.Binz, A.Plückthun, M.G.Grütter, and W.F.van Gunsteren (2006).
Molecular dynamics study of the stabilities of consensus designed ankyrin repeat proteins.
  Proteins, 65, 285-295.  
16329141 R.C.Scarpulla (2006).
Nuclear control of respiratory gene expression in mammalian cells.
  J Cell Biochem, 97, 673-683.  
15656975 D.A.O'Leary, D.Koleski, I.Kola, P.J.Hertzog, and S.Ristevski (2005).
Identification and expression analysis of alternative transcripts of the mouse GA-binding protein (Gabp) subunits alpha and beta1.
  Gene, 344, 79-92.  
15802641 J.Iwahara, R.D.Peterson, and R.T.Clubb (2005).
Compensating increases in protein backbone flexibility occur when the Dead ringer AT-rich interaction domain (ARID) binds DNA: a nitrogen-15 relaxation study.
  Protein Sci, 14, 1140-1150.  
16055742 K.Tsuchimochi, N.Yagishita, S.Yamasaki, T.Amano, Y.Kato, K.Kawahara, S.Aratani, H.Fujita, F.Ji, A.Sugiura, T.Izumi, A.Sugamiya, I.Maruyama, A.Fukamizu, S.Komiya, K.Nishioka, and T.Nakajima (2005).
Identification of a crucial site for synoviolin expression.
  Mol Cell Biol, 25, 7344-7356.  
15707503 R.P.Perry (2005).
The architecture of mammalian ribosomal protein promoters.
  BMC Evol Biol, 5, 15.  
16194281 T.J.Magliery, and L.Regan (2005).
Sequence variation in ligand binding sites in proteins.
  BMC Bioinformatics, 6, 240.  
15798196 W.Hu, A.S.Philips, J.C.Kwok, M.Eisbacher, and B.H.Chong (2005).
Identification of nuclear import and export signals within Fli-1: roles of the nuclear import signals in Fli-1-dependent activation of megakaryocyte-specific promoters.
  Mol Cell Biol, 25, 3087-3108.  
14743390 C.J.Day, M.S.Kim, S.R.Stephens, W.E.Simcock, C.J.Aitken, G.C.Nicholson, and N.A.Morrison (2004).
Gene array identification of osteoclast genes: differential inhibition of osteoclastogenesis by cyclosporin A and granulocyte macrophage colony stimulating factor.
  J Cell Biochem, 91, 303-315.  
15361867 H.H.Xue, J.Bollenbacher, V.Rovella, R.Tripuraneni, Y.B.Du, C.Y.Liu, A.Williams, J.P.McCoy, and W.J.Leonard (2004).
GA binding protein regulates interleukin 7 receptor alpha-chain gene expression in T cells.
  Nat Immunol, 5, 1036-1044.  
14963116 K.Aurrekoetxea-Hernández, and E.Buetti (2004).
Transforming growth factor beta enhances the glucocorticoid response of the mouse mammary tumor virus promoter through Smad and GA-binding proteins.
  J Virol, 78, 2201-2211.  
15152081 L.K.Mosavi, T.J.Cammett, D.C.Desrosiers, and Z.Y.Peng (2004).
The ankyrin repeat as molecular architecture for protein recognition.
  Protein Sci, 13, 1435-1448.  
14978306 P.Gutiérrez, M.J.Osborne, N.Siddiqui, J.F.Trempe, C.Arrowsmith, and K.Gehring (2004).
Structure of the archaeal translation initiation factor aIF2 beta from Methanobacterium thermoautotrophicum: implications for translation initiation.
  Protein Sci, 13, 659-667.
PDB code: 1nee
15100410 V.K.Mootha, C.Handschin, D.Arlow, X.Xie, J.St Pierre, S.Sihag, W.Yang, D.Altshuler, P.Puigserver, N.Patterson, P.J.Willy, I.G.Schulman, R.A.Heyman, E.S.Lander, and B.M.Spiegelman (2004).
Erralpha and Gabpa/b specify PGC-1alpha-dependent oxidative phosphorylation gene expression that is altered in diabetic muscle.
  Proc Natl Acad Sci U S A, 101, 6570-6575.  
15498935 V.S.Devi, H.K.Binz, M.T.Stumpp, A.Plückthun, H.R.Bosshard, and I.Jelesarov (2004).
Folding of a designed simple ankyrin repeat protein.
  Protein Sci, 13, 2864-2870.  
12566564 A.Kohl, H.K.Binz, P.Forrer, M.T.Stumpp, A.Plückthun, and M.G.Grütter (2003).
Designed to be stable: crystal structure of a consensus ankyrin repeat protein.
  Proc Natl Acad Sci U S A, 100, 1700-1705.
PDB code: 1mj0
14592863 A.Méjat, A.Ravel-Chapuis, M.Vandromme, and L.Schaeffer (2003).
Synapse-specific gene expression at the neuromuscular junction.
  Ann N Y Acad Sci, 998, 53-65.  
12756238 E.Lacazette, S.Le Calvez, N.Gajendran, and H.R.Brenner (2003).
A novel pathway for MuSK to induce key genes in neuromuscular synapse formation.
  J Cell Biol, 161, 727-736.  
12646494 H.Iida, A.Urasoko, M.Doiguchi, T.Mōri, K.Toshimori, and Y.Shibata (2003).
Complementary DNA cloning and characterization of rat spergen-2, a spermatogenic cell-specific gene 2 encoding a 56-kilodalton nuclear protein bearing ankyrin repeat motifs.
  Biol Reprod, 69, 421-429.  
14500810 H.Maier, R.Ostraat, S.Parenti, D.Fitzsimmons, L.J.Abraham, C.W.Garvie, and J.Hagman (2003).
Requirements for selective recruitment of Ets proteins and activation of mb-1/Ig-alpha gene transcription by Pax-5 (BSAP).
  Nucleic Acids Res, 31, 5483-5489.  
12581658 K.Ogata, K.Sato, T.H.Tahirov, and T.Tahirov (2003).
Eukaryotic transcriptional regulatory complexes: cooperativity from near and afar.
  Curr Opin Struct Biol, 13, 40-48.  
12517341 K.S.Tang, A.R.Fersht, and L.S.Itzhaki (2003).
Sequential unfolding of ankyrin repeats in tumor suppressor p16.
  Structure, 11, 67-73.  
14573873 M.E.Zweifel, D.J.Leahy, F.M.Hughson, and D.Barrick (2003).
Structure and stability of the ankyrin domain of the Drosophila Notch receptor.
  Protein Sci, 12, 2622-2632.
PDB code: 1ot8
14585962 M.Sunesen, M.Huchet-Dymanus, M.O.Christensen, and J.P.Changeux (2003).
Phosphorylation-elicited quaternary changes of GA binding protein in transcriptional activation.
  Mol Cell Biol, 23, 8008-8018.  
11948622 A.Verger, and M.Duterque-Coquillaud (2002).
When Ets transcription factors meet their partners.
  Bioessays, 24, 362-370.  
12453417 C.R.Escalante, A.L.Brass, J.M.Pongubala, E.Shatova, L.Shen, H.Singh, and A.K.Aggarwal (2002).
Crystal structure of PU.1/IRF-4/DNA ternary complex.
  Mol Cell, 10, 1097-1105.  
11841229 G.M.Poon, P.Gross, and R.B.Macgregor (2002).
The sequence-specific association of the ETS domain of murine PU.1 with DNA exhibits unusual energetics.
  Biochemistry, 41, 2361-2371.  
11884602 L.Hauck, R.G.Kaba, M.Lipp, R.Dietz, and R.von Harsdorf (2002).
Regulation of E2F1-dependent gene transcription and apoptosis by the ETS-related transcription factor GABPgamma1.
  Mol Cell Biol, 22, 2147-2158.  
12142282 M.A.Pufall, and B.J.Graves (2002).
Autoinhibitory domains: modular effectors of cellular regulation.
  Annu Rev Cell Dev Biol, 18, 421-462.  
12456646 P.Michaely, D.R.Tomchick, M.Machius, and R.G.Anderson (2002).
Crystal structure of a 12 ANK repeat stack from human ankyrinR.
  EMBO J, 21, 6387-6396.
PDB code: 1n11
11779502 C.W.Garvie, J.Hagman, and C.Wolberger (2001).
Structural studies of Ets-1/Pax5 complex formation on DNA.
  Mol Cell, 8, 1267-1276.
PDB codes: 1k78 1k79 1k7a
  11156985 D.Rudel, and J.Kimble (2001).
Conservation of glp-1 regulation and function in nematodes.
  Genetics, 157, 639-654.  
11701129 J.Chai, Q.Wu, E.Shiozaki, S.M.Srinivasula, E.S.Alnemri, and Y.Shi (2001).
Crystal structure of a procaspase-7 zymogen: mechanisms of activation and substrate binding.
  Cell, 107, 399-407.
PDB codes: 1k86 1k88
11698648 K.F.Pedley, and J.D.Walton (2001).
Regulation of cyclic peptide biosynthesis in a plant pathogenic fungus by a novel transcription factor.
  Proc Natl Acad Sci U S A, 98, 14174-14179.  
11498047 L.Schaeffer, A.de Kerchove d'Exaerde, and J.P.Changeux (2001).
Targeting transcription to the neuromuscular synapse.
  Neuron, 31, 15-22.  
11463838 N.Nekrep, M.Geyer, N.Jabrane-Ferrat, and B.M.Peterlin (2001).
Analysis of ankyrin repeats reveals how a single point mutation in RFXANK results in bare lymphocyte syndrome.
  Mol Cell Biol, 21, 5566-5576.  
11222776 R.E.Schweppe, and A.Gutierrez-Hartmann (2001).
Pituitary Ets-1 and GABP bind to the growth factor regulatory sites of the rat prolactin promoter.
  Nucleic Acids Res, 29, 1251-1260.  
11257229 T.H.Tahirov, T.Inoue-Bungo, H.Morii, A.Fujikawa, M.Sasaki, K.Kimura, M.Shiina, K.Sato, T.Kumasaka, M.Yamamoto, S.Ishii, and K.Ogata (2001).
Structural analyses of DNA recognition by the AML1/Runx-1 Runt domain and its allosteric control by CBFbeta.
  Cell, 104, 755-767.
PDB codes: 1hjb 1hjc 1io4
11163135 V.Bennett, and L.Chen (2001).
Ankyrins and cellular targeting of diverse membrane proteins to physiological sites.
  Curr Opin Cell Biol, 13, 61-67.  
10762332 C.Zhang, and M.T.Wong-Riley (2000).
Depolarizing stimulation upregulates GA-binding protein in neurons: a transcription factor involved in the bigenomic expression of cytochrome oxidase subunits.
  Eur J Neurosci, 12, 1013-1023.  
  10673508 D.O.Cowley, and B.J.Graves (2000).
Phosphorylation represses Ets-1 DNA binding by reinforcing autoinhibition.
  Genes Dev, 14, 366-376.  
10675337 J.L.Vogel, and T.M.Kristie (2000).
The novel coactivator C1 (HCF) coordinates multiprotein enhancer formation and mediates transcription activation by GABP.
  EMBO J, 19, 683-690.  
10779326 J.Villard, M.Peretti, K.Masternak, E.Barras, G.Caretti, R.Mantovani, and W.Reith (2000).
A functionally essential domain of RFX5 mediates activation of major histocompatibility complex class II promoters by promoting cooperative binding between RFX and NF-Y.
  Mol Cell Biol, 20, 3364-3376.  
10799572 K.Aurrekoetxea-Hernández, and E.Buetti (2000).
Synergistic action of GA-binding protein and glucocorticoid receptor in transcription from the mouse mammary tumor virus promoter.
  J Virol, 74, 4988-4998.  
10606656 P.Dalgleish, and A.D.Sharrocks (2000).
The mechanism of complex formation between Fli-1 and SRF transcription factors.
  Nucleic Acids Res, 28, 560-569.  
10594011 T.L.Goetz, T.L.Gu, N.A.Speck, and B.J.Graves (2000).
Auto-inhibition of Ets-1 is counteracted by DNA binding cooperativity with core-binding factor alpha2.
  Mol Cell Biol, 20, 81-90.  
9890883 A.Scaloni, M.Monti, R.Acquaviva, G.Tell, G.Damante, S.Formisano, and P.Pucci (1999).
Topology of the thyroid transcription factor 1 homeodomain-DNA complex.
  Biochemistry, 38, 64-72.  
  10490644 C.Ducret, S.M.Maira, A.Dierich, and B.Wasylyk (1999).
The net repressor is regulated by nuclear export in response to anisomycin, UV, and heat shock.
  Mol Cell Biol, 19, 7076-7087.  
10410794 C.Wolberger (1999).
Multiprotein-DNA complexes in transcriptional regulation.
  Annu Rev Biophys Biomol Struct, 28, 29-56.  
10541551 D.Chasman, K.Cepek, P.A.Sharp, and C.O.Pabo (1999).
Crystal structure of an OCA-B peptide bound to an Oct-1 POU domain/octamer DNA complex: specific recognition of a protein-DNA interface.
  Genes Dev, 13, 2650-2657.
PDB code: 1cqt
  10548056 F.Pio, N.Assa-Munt, J.Yguerabide, and R.A.Maki (1999).
Mutants of ETS domain PU.1 and GGAA/T recognition: free energies and kinetics.
  Protein Sci, 8, 2098-2109.  
10074345 J.Li, I.J.Byeon, K.Ericson, M.J.Poi, P.O'Maille, T.Selby, and M.D.Tsai (1999).
Tumor suppressor INK4: determination of the solution structure of p18INK4C and demonstration of the functional significance of loops in p18INK4C and p16INK4A.
  Biochemistry, 38, 2930-2940.
PDB code: 1bu9
  9882338 K.Verhoef, R.W.Sanders, V.Fontaine, S.Kitajima, and B.Berkhout (1999).
Evolution of the human immunodeficiency virus type 1 long terminal repeat promoter by conversion of an NF-kappaB enhancer element into a GABP binding site.
  J Virol, 73, 1331-1340.  
10047576 L.Chen (1999).
Combinatorial gene regulation by eukaryotic transcription factors.
  Curr Opin Struct Biol, 9, 48-55.  
10361086 M.R.Groves, and D.Barford (1999).
Topological characteristics of helical repeat proteins.
  Curr Opin Struct Biol, 9, 383-389.  
10103057 M.Wolf-Watz, X.Q.Xie, M.Holm, T.Grundström, and T.Härd (1999).
Solution properties of the free and DNA-bound Runt domain of AML1.
  Eur J Biochem, 261, 251-260.  
9990060 N.Bannert, A.Avots, M.Baier, E.Serfling, and R.Kurth (1999).
GA-binding protein factors, in concert with the coactivator CREB binding protein/p300, control the induction of the interleukin 16 promoter in T lymphocytes.
  Proc Natl Acad Sci U S A, 96, 1541-1546.  
10431175 S.G.Sedgwick, and S.J.Smerdon (1999).
The ankyrin repeat: a diversity of interactions on a common structural framework.
  Trends Biochem Sci, 24, 311-316.  
9874770 S.Simeonidis, D.Stauber, G.Chen, W.A.Hendrickson, and D.Thanos (1999).
Mechanisms by which IkappaB proteins control NF-kappaB activity.
  Proc Natl Acad Sci U S A, 96, 49-54.  
11232330 T.Huxford, S.Malek, and G.Ghosh (1999).
Structure and mechanism in NF-kappa B/I kappa B signaling.
  Cold Spring Harb Symp Quant Biol, 64, 533-540.  
  10022910 W.Wheat, D.Fitzsimmons, H.Lennox, S.R.Krautkramer, L.N.Gentile, L.P.McIntosh, and J.Hagman (1999).
The highly conserved beta-hairpin of the paired DNA-binding domain is required for assembly of Pax-Ets ternary complexes.
  Mol Cell Biol, 19, 2231-2241.  
10075931 W.Y.Kim, M.Sieweke, E.Ogawa, H.J.Wee, U.Englmeier, T.Graf, and Y.Ito (1999).
Mutual activation of Ets-1 and AML1 DNA binding by direct interaction of their autoinhibitory domains.
  EMBO J, 18, 1609-1620.  
10384327 B.J.Graves, D.O.Cowley, T.L.Goetz, J.M.Petersen, M.D.Jonsen, and M.E.Gillespie (1998).
Autoinhibition as a transcriptional regulatory mechanism.
  Cold Spring Harb Symp Quant Biol, 63, 621-629.  
9689043 C.M.Groft, S.N.Uljon, R.Wang, and M.H.Werner (1998).
Structural homology between the Rap30 DNA-binding domain and linker histone H5: implications for preinitiation complex assembly.
  Proc Natl Acad Sci U S A, 95, 9117-9122.
PDB codes: 1bby 2bby
9794820 C.Wolberger (1998).
Combinatorial transcription factors.
  Curr Opin Genet Dev, 8, 552-559.  
9850860 D.H.Chu, C.T.Morita, and A.Weiss (1998).
The Syk family of protein tyrosine kinases in T-cell activation and development.
  Immunol Rev, 165, 167-180.  
9546211 K.R.Ely, and R.Kodandapani (1998).
Ankyrin(g) ETS domains to DNA.
  Nat Struct Biol, 5, 255-259.  
9789010 L.A.Dickinson, R.J.Gulizia, J.W.Trauger, E.E.Baird, D.E.Mosier, J.M.Gottesfeld, and P.B.Dervan (1998).
Inhibition of RNA polymerase II transcription in human cells by synthetic DNA-binding ligands.
  Proc Natl Acad Sci U S A, 95, 12890-12895.  
  9765208 L.Fromm, and S.J.Burden (1998).
Synapse-specific and neuregulin-induced transcription require an ets site that binds GABPalpha/GABPbeta.
  Genes Dev, 12, 3074-3083.  
9782052 R.Baumgartner, C.Fernandez-Catalan, A.Winoto, R.Huber, R.A.Engh, and T.A.Holak (1998).
Structure of human cyclin-dependent kinase inhibitor p19INK4d: comparison to known ankyrin-repeat-containing structures and implications for the dysfunction of tumor suppressor p16INK4a.
  Structure, 6, 1279-1290.
PDB code: 1bd8
9734357 Y.Mo, B.Vaessen, K.Johnston, and R.Marmorstein (1998).
Structures of SAP-1 bound to DNA targets from the E74 and c-fos promoters: insights into DNA sequence discrimination by Ets proteins.
  Mol Cell, 2, 201-212.
PDB codes: 1bc7 1bc8
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.

 

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