PDBsum entry: 1k79

Transcription/DNA

PDB id: 1k79

Contents

Protein chains

104 a.a. *

DNA/RNA

Waters ×96

* Residue conservation analysis

PDB id:

1k79

Name:

Transcription/DNA

Title:

Ets-1(331-440)+ggaa duplex

Structure:

DNA (5'- d( Tp Ap Gp Tp Gp Cp Cp Gp Gp Ap Ap Ap Tp Gp T)-3'). Chain: b, e. Engineered: yes. DNA (5'- d( Cp Ap Cp Ap Tp Tp Tp Cp Cp Gp Gp Cp Ap Cp T)-3'). Chain: c, f. Engineered: yes. C-ets-1 protein.

Source:

Synthetic: yes. Mus musculus. House mouse. Organism_taxid: 10090. Gene: ets-1. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Biol. unit:

Trimer (from PQS)

Resolution:

2.40Å R-factor: 0.224 R-free: 0.279

Authors:

C.W.Garvie,J.Hagman,C.Wolberger

Key ref:

C.W.Garvie et al. (2001). Structural studies of Ets-1/Pax5 complex formation on DNA. Mol Cell, 8, 1267-1276. PubMed id: 11779502 DOI: 10.1016/S1097-2765(01)00410-5

Date:

18-Oct-01 Release date: 04-Jan-02

Protein chains

P27577  (ETS1_MOUSE) -  Protein C-ets-1

Seq: 440 a.a.

Struc: 104 a.a.

 Gene Ontology (GO) functional annotation 

• Biological process: regulation of transcription, DNA-dependent (1 term)
• Biochemical function: transcription factor activity (2 terms)

DOI no: 10.1016/S1097-2765(01)00410-5

Mol Cell 8:1267-1276 (2001)

PubMed id: 11779502

Structural studies of Ets-1/Pax5 complex formation on DNA.

C.W.Garvie, J.Hagman, C.Wolberger.

ABSTRACT

Pax5 regulates the B cell-specific expression of the mb-1 gene together with members of the Ets family of transcriptional activators. The Ets proteins on their own bind poorly to the Pax5/Ets binding site, but can be recruited to the site by cooperative interactions with Pax5. The structure of the ETS domain of Ets-1 and the paired domain of Pax5 bound to DNA reveals the molecular details of the selective recruitment of different Ets proteins by Pax5. Comparison with structures of Ets-1 alone bound to both high- and low-affinity DNA sites reveals that Pax5 alters the Ets-1 contacts with DNA. The ability of one protein to alter the DNA sequence-specific contacts of another provides a general mechanism for combinatorial regulation of transcription.

Selected figure(s)

Figure 3.
Figure 3. Residues Involved in the Interface between Pax5 and Ets-1 and Comparison with the Related Residues from Other Ets Proteins(A) Interface between Pax5 and Ets-1. The residues involved in the interface are labeled with a color denoting whether they came from the ETS domain (purple) or paired domain (blue). Hydrogen bonds and van der Waals contacts are indicated with dashed yellow lines. The DNA contacts made by Tyr395 are not shown in this depiction.(B) Sequence alignment of the ETS domains of Ets-1, Fli-1, GABPα, Net-1, Elk-1 and SAP-1. The secondary structure elements and the residue numbers of the ETS domain of Ets-1 are indicated above the protein sequence. α helices are depicted by rectangles and β strands as arrows. ETS domain residues with the same sequence as Ets-1 are highlighted in yellow. The Ets-1 residues involved in the Pax5/Ets-1 interface are indicated above the protein sequence.

Figure 5.
Figure 5. Conformational Switch of Tyr395 Of Ets-1 Induced by the Presence of Pax5

The above figures are reprinted by permission from Cell Press: Mol Cell (2001, 8, 1267-1276) copyright 2001.