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PDBsum entry 1aa2
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Cytoskeleton PDB id
1aa2

 

 

 

 

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Contents
Protein chain
108 a.a. *
Waters ×130
* Residue conservation analysis
PDB id:
1aa2
Name: Cytoskeleton
Title: Calponin homology (ch) domain from human beta-spectrin
Structure: Beta-spectrin. Chain: a. Fragment: f-actin binding domain residues 173 - 281. Synonym: calponin homology (ch) domain. Engineered: yes
Source: Homo sapiens. Human. Organism_taxid: 9606. Organelle: non-erythrocyte. Expressed in: escherichia coli bl21. Expression_system_taxid: 511693.
Resolution:
2.00Å     R-factor:   0.160     R-free:   0.210
Authors: K.Djinovic Carugo,S.Banuelos,M.Saraste
Key ref: K.Djinovic Carugo et al. (1997). Crystal structure of a calponin homology domain. Nat Struct Biol, 4, 175-179. PubMed id: 9164454
Date:
21-Jan-97     Release date:   04-Feb-98    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Q01082  (SPTB2_HUMAN) -  Spectrin beta chain, non-erythrocytic 1 from Homo sapiens
Seq:
Struc: 		 
Seq:
Struc: 		 
Seq:
Struc: 		 
Seq:
Struc: 		 
Seq:
Struc: 		2364 a.a.
108 a.a.
Key:    Secondary structure  CATH domain

 

 
Nat Struct Biol 4:175-179 (1997)
PubMed id: 9164454  
 
 
Crystal structure of a calponin homology domain.
K.Djinovic Carugo, S.Bañuelos, M.Saraste.
 
  ABSTRACT  
 
The three-dimensional structure of the calponin homology domain present in many actin binding cytoskeletal and signal-transducing proteins has been determined at 2.0 A resolution.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
20668894 A.J.Baines (2010).
The spectrin-ankyrin-4.1-adducin membrane skeleton: adapting eukaryotic cells to the demands of animal life.
  Protoplasma, 244, 99.  
20977743 C.D.White, H.Khurana, D.V.Gnatenko, Z.Li, R.D.Odze, D.B.Sacks, and V.A.Schmidt (2010).
IQGAP1 and IQGAP2 are reciprocally altered in hepatocellular carcinoma.
  BMC Gastroenterol, 10, 125.  
18940607 S.Lorenz, I.Vakonakis, E.D.Lowe, I.D.Campbell, M.E.Noble, and M.K.Hoellerer (2008).
Structural analysis of the interactions between paxillin LD motifs and alpha-parvin.
  Structure, 16, 1521-1531.
PDB codes: 2vzc 2vzd 2vzg 2vzi
18182282 T.U.Tanaka, and A.Desai (2008).
Kinetochore-microtubule interactions: the means to the end.
  Curr Opin Cell Biol, 20, 53-63.  
18180285 V.A.Schmidt, C.S.Chiariello, E.Capilla, F.Miller, and W.F.Bahou (2008).
Development of hepatocellular carcinoma in Iqgap2-deficient mice is IQGAP1 dependent.
  Mol Cell Biol, 28, 1489-1502.  
18508764 X.Wang, K.Fukuda, I.J.Byeon, A.Velyvis, C.Wu, A.Gronenborn, and J.Qin (2008).
The structure of alpha-parvin CH2-paxillin LD1 complex reveals a novel modular recognition for focal adhesion assembly.
  J Biol Chem, 283, 21113-21119.
PDB code: 2k2r
  17401197 A.H.Aguda, A.M.Sakwe, L.Rask, and R.C.Robinson (2007).
Expression, crystallization and preliminary crystallographic data analysis of filamin A repeats 14-16.
  Acta Crystallogr Sect F Struct Biol Cryst Commun, 63, 291-293.  
17222516 C.Antolik, D.H.Catino, A.M.O'Neill, W.G.Resneck, J.A.Ursitti, and R.J.Bloch (2007).
The actin binding domain of ACF7 binds directly to the tetratricopeptide repeat domains of rapsyn.
  Neuroscience, 145, 56-65.  
17553790 T.Keskanokwong, H.J.Shandro, D.E.Johnson, S.Kittanakom, G.L.Vilas, P.Thorner, R.A.Reithmeier, V.Akkarapatumwong, P.T.Yenchitsomanus, and J.R.Casey (2007).
Interaction of integrin-linked kinase with the kidney chloride/bicarbonate exchanger, kAE1.
  J Biol Chem, 282, 23205-23218.  
17043746 H.Sun, H.Dai, J.Zhang, X.Jin, S.Xiong, J.Xu, J.Wu, and Y.Shi (2006).
Solution structure of calponin homology domain of Human MICAL-1.
  J Biomol NMR, 36, 295-300.
PDB code: 2dk9
15635447 T.H.Millard, G.Bompard, M.Y.Heung, T.R.Dafforn, D.J.Scott, L.M.Machesky, and K.Fütterer (2005).
Structural basis of filopodia formation induced by the IRSp53/MIM homology domain of human IRSp53.
  EMBO J, 24, 240-250.
PDB code: 1y2o
15272162 C.H.Wang, M.K.Balasubramanian, and T.Dokland (2004).
Structure, crystal packing and molecular dynamics of the calponin-homology domain of Schizosaccharomyces pombe Rng2.
  Acta Crystallogr D Biol Crystallogr, 60, 1396-1403.
PDB codes: 1p2x 1p5s
15274920 M.G.Klein, W.Shi, U.Ramagopal, Y.Tseng, D.Wirtz, D.R.Kovar, C.J.Staiger, and S.C.Almo (2004).
Structure of the actin crosslinking core of fimbrin.
  Structure, 12, 999.
PDB codes: 1pxy 1rt8
15331592 R.Kristelly, G.Gao, and J.J.Tesmer (2004).
Structural determinants of RhoA binding and nucleotide exchange in leukemia-associated Rho guanine-nucleotide exchange factor.
  J Biol Chem, 279, 47352-47362.
PDB codes: 1txd 1x86
12517699 S.J.Winder (2003).
Structural insights into actin-binding, branching and bundling proteins.
  Curr Opin Cell Biol, 15, 14-22.  
12612583 S.P.Robertson, S.R.Twigg, A.J.Sutherland-Smith, V.Biancalana, R.J.Gorlin, D.Horn, S.J.Kenwrick, C.A.Kim, E.Morava, R.Newbury-Ecob, K.H.Orstavik, O.W.Quarrell, C.E.Schwartz, D.J.Shears, M.Suri, J.Kendrick-Jones, and A.O.Wilkie (2003).
Localized mutations in the gene encoding the cytoskeletal protein filamin A cause diverse malformations in humans.
  Nat Genet, 33, 487-491.  
11790784 B.K.Chauhan, N.A.Reed, W.Zhang, M.K.Duncan, M.W.Kilimann, and A.Cvekl (2002).
Identification of genes downstream of Pax6 in the mouse lens using cDNA microarrays.
  J Biol Chem, 277, 11539-11548.  
11381081 N.Volkmann, D.DeRosier, P.Matsudaira, and D.Hanein (2001).
An atomic model of actin filaments cross-linked by fimbrin and its implications for bundle assembly and function.
  J Cell Biol, 153, 947-956.  
11402068 S.Yamaji, A.Suzuki, Y.Sugiyama, Y.Koide, M.Yoshida, H.Kanamori, H.Mohri, S.Ohno, and Y.Ishigatsubo (2001).
A novel integrin-linked kinase-binding protein, affixin, is involved in the early stage of cell-substrate interaction.
  J Cell Biol, 153, 1251-1264.  
10891857 C.A.Moores, and J.Kendrick-Jones (2000).
Biochemical characterisation of the actin-binding properties of utrophin.
  Cell Motil Cytoskeleton, 46, 116-128.  
10801490 F.L.Norwood, A.J.Sutherland-Smith, N.H.Keep, and J.Kendrick-Jones (2000).
The structure of the N-terminal actin-binding domain of human dystrophin and how mutations in this domain may cause Duchenne or Becker muscular dystrophy.
  Structure, 8, 481-491.
PDB code: 1dxx
10760950 R.A.Zuellig, B.C.Bornhauser, I.Knuesel, F.Heller, J.M.Fritschy, and M.C.Schaub (2000).
Identification and characterisation of transcript and protein of a new short N-terminal utrophin isoform.
  J Cell Biochem, 77, 418-431.  
11134073 S.N.Nikolopoulos, and C.E.Turner (2000).
Actopaxin, a new focal adhesion protein that binds paxillin LD motifs and actin and regulates cell adhesion.
  J Cell Biol, 151, 1435-1448.  
10669724 X.R.Bustelo (2000).
Regulatory and signaling properties of the Vav family.
  Mol Cell Biol, 20, 1461-1477.  
10585942 B.Leinweber, J.X.Tang, W.F.Stafford, and J.M.Chalovich (1999).
Calponin interaction with alpha-actinin-actin: evidence for a structural role for calponin.
  Biophys J, 77, 3208-3217.  
  18521272 H.Ono, H.Yoshikawa, T.Ueda, H.Yamamura, I.Kudawara, M.Manou, S.Ishiguro, H.Funai, Y.Koyanagi, N.Araki, N.Hashimoto, H.Sonobe, M.Tatsuta, and K.Takahashi (1999).
Expression of smooth muscle calponin in synovial sarcoma.
  Sarcoma, 3, 107-113.  
9990286 M.Van Troys, J.Vandekerckhove, and C.Ampe (1999).
Structural modules in actin-binding proteins: towards a new classification.
  Biochim Biophys Acta, 1448, 323-348.  
10647184 N.H.Keep, S.J.Winder, C.A.Moores, S.Walke, F.L.Norwood, and J.Kendrick-Jones (1999).
Crystal structure of the actin-binding region of utrophin reveals a head-to-tail dimer.
  Structure, 7, 1539-1546.
PDB code: 1qag
9631289 A.McGough (1998).
F-actin-binding proteins.
  Curr Opin Struct Biol, 8, 166-176.  
9768350 M.Gimona, and S.J.Winder (1998).
Single calponin homology domains are not actin-binding domains.
  Curr Biol, 8, R674-R675.  
9817844 S.Bañuelos, M.Saraste, and K.Djinović Carugo (1998).
Structural comparisons of calponin homology domains: implications for actin binding.
  Structure, 6, 1419-1431.
PDB code: 1bkr
9484592 Y.A.Puius, N.M.Mahoney, and S.C.Almo (1998).
The modular structure of actin-regulatory proteins.
  Curr Opin Cell Biol, 10, 23-34.  
9353348 I.N.Rybakova, and J.M.Ervasti (1997).
Dystrophin-glycoprotein complex is monomeric and stabilizes actin filaments in vitro through a lateral association.
  J Biol Chem, 272, 28771-28778.  
9305904 J.W.Erickson, R.A.Cerione, and M.J.Hart (1997).
Identification of an actin cytoskeletal complex that includes IQGAP and the Cdc42 GTPase.
  J Biol Chem, 272, 24443-24447.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.

 

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