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PDBsum entry 1mee
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protein metals Protein-protein interface(s) links
Complex(serine proteinase-inhibitor) PDB id
1mee

 

 

 

 

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Contents
Protein chains
275 a.a. *
64 a.a. *
Metals
_CA ×2
Waters ×312
* Residue conservation analysis
PDB id:
1mee
Name: Complex(serine proteinase-inhibitor)
Title: The complex between the subtilisin from a mesophilic bacterium and the leech inhibitor eglin-c
Structure: Mesentericopeptidase. Chain: a. Engineered: yes. Eglin c. Chain: i. Engineered: yes
Source: Bacillus pumilus. Organism_taxid: 1408. Hirudo medicinalis. Medicinal leech. Organism_taxid: 6421
Biol. unit: Dimer (from PQS)
Resolution:
2.00Å     R-factor:   0.151    
Authors: Z.Dauter,C.Betzel,K.S.Wilson
Key ref: Z.Dauter et al. (1991). Complex between the subtilisin from a mesophilic bacterium and the leech inhibitor eglin-C. Acta Crystallogr B, 47, 707-730. PubMed id: 1793542
Date:
15-Apr-91     Release date:   15-Oct-92    
PROCHECK
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 Headers
 References

Protein chain
Pfam   ArchSchema ?
P07518  (SUBT_BACPU) -  Subtilisin from Bacillus pumilus
Seq:
Struc: 		275 a.a.
275 a.a.*
Protein chain
Pfam   ArchSchema ?
P01051  (ICIC_HIRME) -  Eglin C from Hirudo medicinalis
Seq:
Struc: 		70 a.a.
64 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 3 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class 2: Chain A: E.C.3.4.21.62  - subtilisin.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyzes peptide amides.
   Enzyme class 3: Chain I: E.C.?
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

 

 
Acta Crystallogr B 47:707-730 (1991)
PubMed id: 1793542  
 
 
Complex between the subtilisin from a mesophilic bacterium and the leech inhibitor eglin-C.
Z.Dauter, C.Betzel, N.Genov, N.Pipon, K.S.Wilson.
 
  ABSTRACT  
 
The alkaline proteinase from the mesophilic bacterium Bacillus mesentericus has been crystallized in a 1:1 complex with the inhibitor eglin-C from the medical leech. The crystals have cell dimensions of a = 43.0, b = 71.9, c = 48.3 A and beta = 110.0 degrees and are in the space group P2(1). Three-dimensional data to 2.0 A have been recorded on film from a single crystal. The orientation and position of the complex in the unit cell have been established using the refined coordinates of subtilisin Carlsberg and of eglin-C as independent models. The structure of the complex has been refined by restrained least-squares minimization. The crystallographic R factor (= sigma[magnitude of Fo - magnitude of Fc[/sigma magnitude of Fo) is 15.1% including two Ca2+ ions and 312 water molecules. The structure is discussed in terms of its physicochemical properties in solution and its relation to other Bacillus subtilisins.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
20541512 J.Vévodová, M.Gamble, G.Künze, A.Ariza, E.Dodson, D.D.Jones, and K.S.Wilson (2010).
Crystal structure of an intracellular subtilisin reveals novel structural features unique to this subtilisin family.
  Structure, 18, 744-755.
PDB codes: 2wv7 2wwt 2x8j
16342264 J.S.Fetrow, S.T.Knutson, and M.H.Edgell (2006).
Mutations in alpha-helical solvent-exposed sites of eglin c have long-range effects: evidence from molecular dynamics simulations.
  Proteins, 63, 356-372.  
15906321 A.J.Bordner, and R.Abagyan (2005).
Statistical analysis and prediction of protein-protein interfaces.
  Proteins, 60, 353-366.  
16344483 C.K.Brown, Z.Y.Gu, Y.V.Matsuka, S.S.Purushothaman, L.A.Winter, P.P.Cleary, S.B.Olmsted, D.H.Ohlendorf, and C.A.Earhart (2005).
Structure of the streptococcal cell wall C5a peptidase.
  Proc Natl Acad Sci U S A, 102, 18391-18396.
PDB code: 1xf1
8913681 J.P.Turkenburg, and E.J.Dodson (1996).
Modern developments in molecular replacement.
  Curr Opin Struct Biol, 6, 604-610.  
7925366 G.Lange, C.Betzel, S.Branner, and K.S.Wilson (1994).
Crystallographic studies of Savinase, a subtilisin-like proteinase, at pH 10.5.
  Eur J Biochem, 224, 507-518.  
7922044 K.Huang, N.C.Strynadka, V.D.Bernard, R.J.Peanasky, and M.N.James (1994).
The molecular structure of the complex of Ascaris chymotrypsin/elastase inhibitor with porcine elastase.
  Structure, 2, 679-689.
PDB code: 1eai
  1304887 E.Meyer (1992).
Internal water molecules and H-bonding in biological macromolecules: a review of structural features with functional implications.
  Protein Sci, 1, 1543-1562.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.

 

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