PDBsum entry 6qwf

DNA binding protein

PDB id: 6qwf

Contents

Protein chains

236 a.a.

DNA/RNA

Ligands

GOL ×2

Waters ×24

PDB id:

6qwf

Name:

DNA binding protein

Title:

The transcriptional regulator prfa-a94v mutant from listeria monocytogenes in complex with a 30-bp operator prfa-box motif

Structure:

Listeriolysin positive regulatory factor a. Chain: a, b. Synonym: listeriolysin positive regulatory protein,listeriolysin regulatory protein,listeriolysin transcriptional regulator prfa, pleitrophic regulatory factor a,positive regulatory factor a,prfa. Engineered: yes. DNA (30-mer). Chain: c. Engineered: yes.

Source:

Listeria monocytogenes. Organism_taxid: 1639. Gene: prfa, cdr86_15285, d3b95_15085, doe67_09655. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Organism_taxid: 1639

Resolution:

2.70Å R-factor: 0.227 R-free: 0.265

Authors:

M.Hall,C.Grundstrom,S.Hansen,K.Brannstrom,J.Johansson,A.E.Sauer- Eriksson

Date:

05-Mar-19 Release date: 01-Apr-20

Protein chains

P22262  (PRFA_LISMO) -  Listeriolysin regulatory protein from Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)

Seq: 237 a.a.

Struc: 236 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

DNA/RNA chains

T-T-G-A-G-G-C-A-T-T-A-A-C-A-T-T-T-G-T-T-A-A-C-G-A-C-G-A-T-A 30 bases

T-A-T-C-G-T-C-G-T-T-A-A-C-A-A-A-T-G-T-T-A-A-T-G-C-C-T-C-A-A 30 bases

Enzyme reactions

• Enzyme class: E.C.?