PDBsum entry 6pw2

Viral protein/DNA

PDB id: 6pw2

Contents

Protein chains

(+ 2 more) 147 a.a.

DNA/RNA

Waters ×4

PDB id:

6pw2

Name:

Viral protein/DNA

Title:

Structural basis for cooperative binding of ebna1 to the epstein-barr virus dyad symmetry minimal origin of replication

Structure:

Epstein-barr nuclear antigen 1. Chain: a, b, c, d, i, j, k, l. Synonym: ebv nuclear antigen 1. Engineered: yes. DNA (62-mer). Chain: e, g. Engineered: yes. Other_details: ctaaccctaattcaatagcatatgttacccaacgggaagcatatgctatcgaattagggtta.

Source:

Epstein-barr virus (strain b95-8). Hhv-4. Organism_taxid: 10377. Strain: b95-8. Gene: ebna1, bkrf1. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Synthetic: yes. Human herpesvirus 4 strain b95-8.

Resolution:

3.01Å R-factor: 0.220 R-free: 0.277

Authors:

T.E.Messick,K.A.Malecka,P.M.Lieberman

Key ref:

K.A.Malecka et al. Structural basis for cooperative binding of ebna1 to epstein-Barr virus dyad symmetry minimal origin of replication.. J virol, . PubMed id: 31142669

Date:

22-Jul-19 Release date: 28-Aug-19

Protein chains

P03211  (EBNA1_EBVB9) -  Epstein-Barr nuclear antigen 1 from Epstein-Barr virus (strain B95-8)

Seq: 641 a.a.

Struc: 147 a.a.

DNA/RNA chains

A-C-C-C-T-A-A-T-T-C-G-A-T-A-G-C-A-T-A-T-G-C-T-T-C-C-C-G-T-T-G-G-G-T-A-A-C-A-T- 57 bases

C-C-T-A-A-T-T-C-A-A-T-A-G-C-A-T-A-T-G-T-T-A-C-C-C-A-A-C-G-G-G-A-A-G-C-A-T-A-T- 53 bases

A-A-T-T-C-G-A-T-A-G-C-A-T-A-T-G-C-T-T-C-C-C-G-T-T-G-G-G-T-A-A-C-A-T-A-T-G-C-T- 49 bases

T-A-A-T-T-C-A-A-T-A-G-C-A-T-A-T-G-T-T-A-C-C-C-A-A-C-G-G-G-A-A-G-C-A-T-A-T-G-C- 49 bases