PDBsum entry 6p8h

Cell cycle, transferase

PDB id: 6p8h

Contents

Protein chains

244 a.a.

258 a.a.

54 a.a.

PDB id:

6p8h

Name:

Cell cycle, transferase

Title:

Crystal structure of cdk4 in complex with cyclind1 and p21

Structure:

G1/s-specific cyclin-d1. Chain: a. Synonym: b-cell lymphoma 1 protein,bcl-1,bcl-1 oncogene,prad1 oncogene. Engineered: yes. Cyclin-dependent kinase 4. Chain: b. Synonym: cell division protein kinase 4,psk-j3. Engineered: yes.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: ccnd1, bcl1, prad1. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Gene: cdk4. Gene: cdkn1a, cap20, cdkn1, cip1, mda6, pic1, sdi1, waf1. Expression_system_taxid: 7108

Resolution:

3.19Å R-factor: 0.208 R-free: 0.258

Authors:

K.Z.Guiley,J.W.Stevenson,K.Lou,K.J.Barkovich,K.Bunch,S.M.Tripathi, K.M.Shokat,S.M.Rubin

Key ref:

K.Z.Guiley et al. (2019). P27 allosterically activates cyclin-Dependent kinase antagonizes palbociclib inhibition.. Science, 366, . PubMed id: 31831640

Date:

07-Jun-19 Release date: 25-Dec-19

Protein chain

P24385  (CCND1_HUMAN) -  G1/S-specific cyclin-D1 from Homo sapiens

Seq: 295 a.a.

Struc: 244 a.a.

Protein chain

P11802  (CDK4_HUMAN) -  Cyclin-dependent kinase 4 from Homo sapiens

Seq: 303 a.a.

Struc: 258 a.a.*

Protein chain

P38936  (CDN1A_HUMAN) -  Cyclin-dependent kinase inhibitor 1 from Homo sapiens

Seq: 164 a.a.

Struc: 54 a.a.

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chain B: E.C.2.7.11.22 - cyclin-dependent kinase.
• Reaction:
  1. L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H⁺
  2. L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site