PDBsum entry 6ejl

Signaling protein

PDB id: 6ejl

Contents

Protein chains

225 a.a.

Ligands

SER-ILE-SEP-LEU-PRO-VAL

SER-ILE-SEP-LEU-PRO-VAL-PRO

Waters ×147

PDB id:

6ejl

Name:

Signaling protein

Title:

Structure of 14-3-3 zeta in complex with ask1 14-3-3 binding motif

Structure:

14-3-3 protein zeta/delta. Chain: a, b. Synonym: protein kinasE C inhibitor protein 1,kcip-1. Engineered: yes. Mitogen-activated protein kinase kinase kinase 5. Chain: c, d. Fragment: unp residues 963-970. Synonym: apoptosis signal-regulating kinase 1,ask-1,mapk/erk kinase kinase 5,mekk 5.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: ywhaz. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Synthetic: yes. Organism_taxid: 9606

Resolution:

2.38Å R-factor: 0.197 R-free: 0.215

Authors:

O.Petrvalska,D.Lentini Santo,V.Obsilova,T.Obsil

Key ref:

O.Petrvalska et al. Crystal structure of 14-3-3 zeta in complex with ask1 binding motif. To be published, .

Date:

21-Sep-17 Release date: 08-Nov-17

Protein chains

P63104  (1433Z_HUMAN) -  14-3-3 protein zeta/delta from Homo sapiens

Seq: 245 a.a.

Struc: 225 a.a.

Enzyme reactions

• Enzyme class: E.C.2.7.11.25 - mitogen-activated protein kinase kinase kinase.
• Reaction:
  1. L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H⁺
  2. L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H⁺

L-seryl-[protein] + ATP (Bound ligand (Het Group name = PRO) matches with 50.00% similarity) = O-phospho-L-seryl-[protein] + ADP + H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site