PDBsum entry 5n4v

Transferase

PDB id: 5n4v

Contents

Protein chain

273 a.a.

Ligands

ARG-LYS-ARG-ARG-ARG-HIS-PRO-SER-GLY

8MW

GOL

Waters ×158

PDB id:

5n4v

Name:

Transferase

Title:

Crystal structure of human pim-1 kinase in complex with a consensuspeptide and fragment like molekule 2-cyclopropyl-4,5- dimethylthieno[5,4-d]pyrimidine-6-carboxylic acid

Structure:

Serine/threonine-protein kinase pim-1. Chain: a. Engineered: yes. Mutation: yes. Other_details: isofrom 2 of pim-1 kinase. Pimtide. Chain: d. Engineered: yes. Other_details: pim-1 consensus peptide

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: pim1. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Expression_system_variant: plyss. Synthetic: yes. Organism_taxid: 9606

Resolution:

1.85Å R-factor: 0.165 R-free: 0.187

Authors:

C.Siefker,A.Heine,G.Klebe

Key ref:

C.Siefker et al. A crystallographic fragment study with human pim-1 ki. To be published, .

Date:

11-Feb-17 Release date: 28-Feb-18

Protein chain

P11309  (PIM1_HUMAN) -  Serine/threonine-protein kinase pim-1 from Homo sapiens

Seq: 313 a.a.

Struc: 273 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.2.7.11.1 - non-specific serine/threonine protein kinase.
• Reaction:
  1. L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H⁺
  2. L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site