PDBsum entry 4xax

Transcription regulator

PDB id: 4xax

Contents

Protein chains

184 a.a.

158 a.a.

Ligands

EDO ×5

Waters ×76

PDB id:

4xax

Name:

Transcription regulator

Title:

Crystal structure of thermus thermophilus card in complex with the thermus aquaticus RNA polymerase beta1 domain

Structure:

DNA-directed RNA polymerase subunit beta domain 1. Chain: a. Synonym: rnap subunit beta,RNA polymerase subunit beta,transcriptase subunit beta,rnap subunit beta,RNA polymerase subunit beta, transcriptase subunit beta. Engineered: yes. Other_details: beta1 domain (unp residues 17-139 and 334-395). Card. Chain: b.

Source:

Thermus aquaticus. Organism_taxid: 271. Gene: rpob. Expressed in: escherichia coli. Expression_system_taxid: 562. Thermus thermophilus (strain hb8 / atcc 27634 / dsm 579). Organism_taxid: 300852. Strain: hb8 / atcc 27634 / dsm 579.

Resolution:

2.40Å R-factor: 0.162 R-free: 0.180

Authors:

J.Chen,B.Bae,E.A.Campbell,S.A.Darst

Key ref:

B.Bae et al. (2015). Card uses a minor groove wedge mechanism to stabilize RNA polymerase open promoter complex.. Elife, 4, . PubMed id: 26349034

Date:

15-Dec-14 Release date: 16-Sep-15

Protein chain

Q9KWU7  (RPOB_THEAQ) -  DNA-directed RNA polymerase subunit beta from Thermus aquaticus

Seq: 1119 a.a.

Struc: 184 a.a.*

Protein chain

Q5SLX5  (Q5SLX5_THET8) -  CarD-like/TRCF RNAP-interacting domain-containing protein from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)

Seq: 164 a.a.

Struc: 158 a.a.

* PDB and UniProt seqs differ at 54 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chain A: E.C.2.7.7.6 - DNA-directed Rna polymerase.
• Reaction: RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site