PDBsum entry 4ger

Hydrolase

PDB id: 4ger

Contents

Protein chains

304 a.a.

Ligands

THR-LYS ×2

Metals

_ZN ×2

_CA ×6

Waters ×612

PDB id:

4ger

Name:

Hydrolase

Title:

Crystal structure of gentlyase, the neutral metalloprotease of paenibacillus polymyxa

Structure:

Gentlyase metalloprotease. Chain: a, b. Engineered: yes

Source:

Paenibacillus polymyxa. Organism_taxid: 1406. Expressed in: bacillus amyloliquefaciens. Expression_system_taxid: 1390

Resolution:

1.59Å R-factor: 0.202 R-free: 0.243

Authors:

A.Ruf,M.Stihle,J.Benz,M.Schmidt,H.Sobek

Key ref:

A.Ruf et al. (2013). Structure of gentlyase, the neutral metalloprotease of Paenibacillus polymyxa. Acta Crystallogr D Biol Crystallogr, 69, 24-31. PubMed id: 23275160

Date:

02-Aug-12 Release date: 02-Jan-13

Protein chains

P29148  (NPRE_PAEPO) -  Bacillolysin from Paenibacillus polymyxa

Seq: 590 a.a.

Struc: 304 a.a.*

* PDB and UniProt seqs differ at 12 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.4.24.28 - bacillolysin.
• Reaction: Similar, but not identical, to that of thermolysin.
• Cofactor: Ca(2+); Zn(2+)

Acta Crystallogr D Biol Crystallogr 69:24-31 (2013)

PubMed id: 23275160

Structure of gentlyase, the neutral metalloprotease of Paenibacillus polymyxa.

A.Ruf, M.Stihle, J.Benz, M.Schmidt, H.Sobek.

ABSTRACT

No abstract given.