EC - Bacillolysin

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IntEnz Enzyme Nomenclature


Accepted name:
Other names:
Bacillus subtilis neutral proteinase
Bacillus metalloendopeptidase
Bacillus metalloproteinase
Bacillus neutral proteinase
anilozyme P 10
Systematic name:




Variants of this enzyme have been found in species of Bacillus including B. subtilis [1,6], B. amyloliquefaciens [5], B. megaterium (megateriopeptidase, [2]), B. mesentericus [10], B. cereus [3,8,9] and B. stearothermophilus [7]. In peptidase family M4 (thermolysin family). Formerly included in EC

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , MEROPS , UniPathway
Protein domains and families: PROSITE:PDOC00129
Structural data: CSA , EC2PDB
CAS Registry Number: 76774-43-1
UniProtKB/Swiss-Prot: (10) [show] [UniProt]


  1. Morihara, K., Tsuzuki, H. and Oka, T.
    Comparison of the specificities of various neutral proteinases from microorganisms.
    Arch. Biochem. Biophys. 123: 572-588 (1968). [PMID: 4967801]
  2. Millet, J. and Acher, R.
    Spécificité de la mégatériopeptidase: une amino-endopeptidase à caractère hydrophobe.
    Eur. J. Biochem. 9: 456-462 (1969). [PMID: 4980359]
  3. Feder, J., Keay, L., Garrett, L.R., Cirulis, N., Moseley, M.H. and Wildi, B.S.
    Bacillus cereus neutral protease.
    Biochim. Biophys. Acta 251: 74-78 (1971). [PMID: 5002444]
  4. Holmquist, B. and Vallee, B.L.
    Esterase activity of zinc neutral proteases.
    Biochemistry 15: 101-107 (1976). [PMID: 2276]
  5. Vasantha, N., Thompson, L.D., Rhodes, C., Banner, C., Nagle, J. and Filpula, D.
    Genes for alkaline protease and neutral protease from Bacillus amyloliquefaciens contain a large open reading frame between the regions coding for signal sequence and mature protein.
    J. Bacteriol. 159: 811-819 (1984). [PMID: 6090391]
  6. Yang, M.Y., Ferrari, E. and Henner, D.J.
    Cloning of the neutral protease gene of Bacillus subtilis and the use of the cloned gene to create an in vitro-derived deletion mutation.
    J. Bacteriol. 160: 15-21 (1984). [PMID: 6090407]
  7. Takagi, M., Imanaka, T. and Aiba, S.
    Nucleotide sequence and promoter region for the neutral protease gene from Bacillus stearothermophilus.
    J. Bacteriol. 163: 824-831 (1985). [PMID: 2993245]
  8. Sidler, W., Niederer, E., Suter, F. and Zuber, H.
    The primary structure of Bacillus cereus neutral proteinase and comparison with thermolysin and Bacillus subtilis neutral proteinase.
    Biol. Chem. Hoppe-Seyler 367: 643-657 (1986). [PMID: 3092843]
  9. Pauptit, R.A., Karlson, R., Picot, D., Jenkins, J.A., Niklaus-Reimer, A.-S. and Jansonius, J.N.
    Crystal structure of neutral protease from Bacillus cereus refined at 3.0 Å resolution and comparison with the homologous but more thermostable enzyme thermolysin.
    J. Mol. Biol. 199: 525-537 (1988). [PMID: 3127592]
  10. Stoeva, S., Kleinschmidt, T., Mesrob, B. and Braunitzer, G.
    Primary structure of a zinc protease from Bacillus mesentericus strain 76.
    Biochemistry 29: 527-534 (1990). [PMID: 2302386]

[EC created 1972 as EC, part transferred 1992 to EC]