PDBsum entry 4f2s

Transferase/DNA

PDB id: 4f2s

Contents

Protein chains

578 a.a.

DNA/RNA

Ligands

GLC-FRU ×2

CTP ×2

SO4

Metals

_MN

Waters ×1323

PDB id:

4f2s

Name:

Transferase/DNA

Title:

DNA polymerase i large fragment complex 4

Structure:

DNA polymerase. Chain: a, d. Fragment: unp residues 287-878. Engineered: yes. Mutation: yes. DNA (5'-d( Cp Cp Tp Gp Ap Cp Tp Cp (Doc))-3'). Chain: b, e. Engineered: yes. Other_details: DNA primer strand.

Source:

Geobacillus kaustophilus. Organism_taxid: 235909. Gene: pola, gk2730. Expressed in: escherichia coli. Expression_system_taxid: 469008. Synthetic: yes. Synthetic construct. Organism_taxid: 32630. Organism_taxid: 32630

Resolution:

1.65Å R-factor: 0.175 R-free: 0.199

Authors:

W.Wang,L.S.Beese

Key ref:

W.Wang et al. Structures of a high-Fidelity DNA polymerase. To be published, .

Date:

08-May-12 Release date: 24-Jul-13

Protein chains

Q5KWC1  (Q5KWC1_GEOKA) -  DNA polymerase I from Geobacillus kaustophilus (strain HTA426)

Seq: 878 a.a.

Struc: 578 a.a.*

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

DNA/RNA chains

C-C-T-G-A-C-T-C-DOC 9 bases

T-G-G-G-A-G-T-C-A-G-G 11 bases

C-C-T-G-A-C-T-C-DOC 9 bases

A-T-G-G-G-A-G-T-C-A-G-G 12 bases

Enzyme reactions

• Enzyme class: E.C.2.7.7.7 - DNA-directed Dna polymerase.
• Reaction: DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site