PDBsum entry 4ed8

Transferase/DNA

PDB id: 4ed8

Contents

Protein chain

430 a.a.

DNA/RNA

Ligands

GOL ×2

DTP

Metals

_MG ×2

_CA

Waters ×479

PDB id:

4ed8

Name:

Transferase/DNA

Title:

Human DNA polymerase eta - DNA ternary complex: reaction in the tg crystal at ph 7.0, normal translocation

Structure:

DNA polymerase eta. Chain: a. Fragment: catalytic core (unp residues 1-432). Synonym: rad30 homolog a, xeroderma pigmentosum variant type protein. Engineered: yes. DNA (5'-d( Cp Ap Tp Tp Ap Tp Gp Ap Cp Gp Cp G)-3'). Chain: t. Engineered: yes. DNA (5'-d( Tp Gp Cp Gp Tp Cp Ap Tp A)-3').

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: polh, rad30, rad30a, xpv. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Synthetic: yes

Resolution:

1.52Å R-factor: 0.173 R-free: 0.200

Authors:

T.Nakamura,Y.Zhao,W.Yang

Key ref:

T.Nakamura et al. (2012). Watching DNA polymerase η make a phosphodiester bond. Nature, 487, 196-201. PubMed id: 22785315

Date:

27-Mar-12 Release date: 11-Jul-12

Protein chain

Q9Y253  (POLH_HUMAN) -  DNA polymerase eta from Homo sapiens

Seq: 713 a.a.

Struc: 430 a.a.

DNA/RNA chains

A-T-T-A-T-G-A-C-G-C-G 11 bases

T-G-C-G-T-C-A-T-A 9 bases

Enzyme reactions

• Enzyme class: E.C.2.7.7.7 - DNA-directed Dna polymerase.
• Reaction: DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

Nature 487:196-201 (2012)

PubMed id: 22785315

Watching DNA polymerase η make a phosphodiester bond.

T.Nakamura, Y.Zhao, Y.Yamagata, Y.J.Hua, W.Yang.

ABSTRACT

No abstract given.