PDBsum entry 3nqy

Hydrolase

PDB id

3nqy

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Contents

			Protein chains

					307 a.a. *


					174 a.a. *

			Metals

					_ZN


					_CA

			Waters ×125

* Residue conservation analysis

PDB id:

3nqy

Links

Name:

Hydrolase

Title:

Crystal structure of the autoprocessed complex of vibriolysin mcp-02 with a single point mutation e346a

Structure:

Secreted metalloprotease mcp02. Chain: b. Fragment: the catalytic domain, residues 205-519. Synonym: mcp-02. Engineered: yes. Mutation: yes. Secreted metalloprotease mcp02. Chain: a. Fragment: propeptide domain, residues 25-204.

Source:

Pseudoalteromonas sp.. Organism_taxid: 234831. Strain: sm9913. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

2.60Å

R-factor:

0.199

R-free:

0.250

Authors:

X.Gao,J.Wang,J.-W.Wu,Y.-Z.Zhang

Key ref:

X.Gao et al. (2010). Structural basis for the autoprocessing of zinc metalloproteases in the thermolysin family. Proc Natl Acad Sci U S A, 107, 17569-17574. PubMed id: 20876133

Date:

30-Jun-10

Release date:

06-Oct-10

PROCHECK

	Headers

	References

Protein chain

A1DRD5 (A1DRD5_PSEU9) - Secreted metalloprotease Mcp02 from Pseudoalteromonas sp. (strain SM9913)

Seq: Struc:

Seq: Struc:					727 a.a. 307 a.a.*

Protein chain

A1DRD5 (A1DRD5_PSEU9) - Secreted metalloprotease Mcp02 from Pseudoalteromonas sp. (strain SM9913)

Seq: Struc:

Seq: Struc:					727 a.a. 174 a.a.

Key:

PfamA domain

Secondary structure

CATH domain

* PDB and UniProt seqs differ at 1 residue position (black cross)



		Enzyme reactions

Enzyme class:

Chains B, A: E.C.3.4.24.25 - vibriolysin.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Reaction:

Preferential cleavage of bonds with bulky hydrophobic groups in P2 and P1'.

Cofactor:

Zn(2+)

	Proc Natl Acad Sci U S A 107:17569-17574 (2010)
PubMed id: 20876133

Structural basis for the autoprocessing of zinc metalloproteases in the thermolysin family.
X.Gao, J.Wang, D.Q.Yu, F.Bian, B.B.Xie, X.L.Chen, B.C.Zhou, L.H.Lai, Z.X.Wang, J.W.Wu, Y.Z.Zhang.

ABSTRACT

No abstract given.

Literature references that cite this PDB file's key reference

PubMed id

Reference

23275160

A.Ruf, M.Stihle, J.Benz, M.Schmidt, and H.Sobek (2013).
Structure of Gentlyase, the neutral metalloprotease of Paenibacillus polymyxa.

Acta Crystallogr D Biol Crystallogr, 69, 24-31.

PDB codes:

4b52 4ger

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.