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PDBsum entry 3nqy

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protein metals Protein-protein interface(s) links
Hydrolase PDB id
3nqy
Jmol
Contents
Protein chains
307 a.a. *
174 a.a. *
Metals
_ZN
_CA
Waters ×125
* Residue conservation analysis
PDB id:
3nqy
Name: Hydrolase
Title: Crystal structure of the autoprocessed complex of vibriolysi with a single point mutation e346a
Structure: Secreted metalloprotease mcp02. Chain: b. Fragment: the catalytic domain, residues 205-519. Synonym: mcp-02. Engineered: yes. Mutation: yes. Secreted metalloprotease mcp02. Chain: a. Fragment: propeptide domain, residues 25-204.
Source: Pseudoalteromonas sp.. Organism_taxid: 234831. Strain: sm9913. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Resolution:
2.60Å     R-factor:   0.199     R-free:   0.250
Authors: X.Gao,J.Wang,J.-W.Wu,Y.-Z.Zhang
Key ref: X.Gao et al. (2010). Structural basis for the autoprocessing of zinc metalloproteases in the thermolysin family. Proc Natl Acad Sci U S A, 107, 17569-17574. PubMed id: 20876133
Date:
30-Jun-10     Release date:   06-Oct-10    
PROCHECK
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 Headers
 References

Protein chain
Pfam   ArchSchema ?
A1DRD5  (A1DRD5_PSEU9) -  Secreted metalloprotease Mcp02
Seq:
Struc:
 
Seq:
Struc:
727 a.a.
307 a.a.*
Protein chain
Pfam   ArchSchema ?
A1DRD5  (A1DRD5_PSEU9) -  Secreted metalloprotease Mcp02
Seq:
Struc:
 
Seq:
Struc:
727 a.a.
174 a.a.
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 1 residue position (black cross)

 Enzyme reactions 
   Enzyme class: Chains B, A: E.C.3.4.24.25  - Vibriolysin.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Preferential cleavage of bonds with bulky hydrophobic groups in P2 and P1'.
      Cofactor: Zn(2+)
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     proteolysis   1 term 
  Biochemical function     metalloendopeptidase activity     1 term  

 

 
Proc Natl Acad Sci U S A 107:17569-17574 (2010)
PubMed id: 20876133  
 
 
Structural basis for the autoprocessing of zinc metalloproteases in the thermolysin family.
X.Gao, J.Wang, D.Q.Yu, F.Bian, B.B.Xie, X.L.Chen, B.C.Zhou, L.H.Lai, Z.X.Wang, J.W.Wu, Y.Z.Zhang.
 
  ABSTRACT  
 
No abstract given.

 

Literature references that cite this PDB file's key reference

  PubMed id Reference
23275160 A.Ruf, M.Stihle, J.Benz, M.Schmidt, and H.Sobek (2013).
Structure of Gentlyase, the neutral metalloprotease of Paenibacillus polymyxa.
  Acta Crystallogr D Biol Crystallogr, 69, 24-31.
PDB codes: 4b52 4ger
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