PDBsum entry 3ndh

Hydrolase/DNA

PDB id: 3ndh

Contents

Protein chains

214 a.a.

DNA/RNA

Ligands

PGE ×3

ACT ×4

SO4 ×2

Metals

_NA ×2

_CL ×2

Waters ×471

PDB id:

3ndh

Name:

Hydrolase/DNA

Title:

Restriction endonuclease in complex with substrate DNA

Structure:

Restriction endonuclease thai. Chain: a, b. Synonym: putative uncharacterized protein ta0828. Engineered: yes. DNA (5'-d( G Gp Tp Ap Cp Gp Cp Gp Ap Tp G)-3'). Chain: c. Engineered: yes. DNA (5'-d( C Cp Ap Tp Cp Gp Cp Gp Tp Ap C)-3'). Chain: d.

Source:

Thermoplasma acidophilum. Organism_taxid: 2303. Strain: atcc25905. Gene: ta0828. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Synthetic: yes

Resolution:

1.30Å R-factor: 0.172 R-free: 0.183

Authors:

M.Firczuk,M.Wojciechowski,H.Czapinska,M.Bochtler

Key ref:

M.Firczuk et al. (2011). DNA intercalation without flipping in the specific ThaI-DNA complex. Nucleic Acids Res, 39, 744-754. PubMed id: 20861000

Date:

07-Jun-10 Release date: 28-Jul-10

Protein chains

Q9HJY3  (Q9HJY3_THEAC) -  Type II restriction endonuclease subunit R from Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)

Seq: 216 a.a.

Struc: 214 a.a.*

* PDB and UniProt seqs differ at 6 residue positions (black crosses)

DNA/RNA chains

G-T-A-C-G-C-G-A-T-G 10 bases

C-A-T-C-G-C-G-T-A-C 10 bases

Enzyme reactions

• Enzyme class: E.C.?

Nucleic Acids Res 39:744-754 (2011)

PubMed id: 20861000

DNA intercalation without flipping in the specific ThaI-DNA complex.

M.Firczuk, M.Wojciechowski, H.Czapinska, M.Bochtler.

ABSTRACT

No abstract given.