PDBsum entry 3m8s

Transferase/DNA

PDB id: 3m8s

Contents

Protein chain

539 a.a. *

DNA/RNA

Ligands

HXB

GOL ×3

PEG

15P

ACT ×4

Metals

_MG ×2

Waters ×122

* Residue conservation analysis

PDB id:

3m8s

Name:

Transferase/DNA

Title:

Crystal structure of the large fragment of DNA polymerase i from thermus aquaticus in a closed ternary complex with trapped 4'- methylated dttp

Structure:

DNA polymerase i, thermostable. Chain: a. Fragment: klenow fragment. Synonym: taq polymerase 1. Engineered: yes. DNA (5'-d( Gp Ap Cp Cp Ap Cp Gp Gp Cp Gp Cp (Doc))-3'). Chain: b. Engineered: yes. DNA (5'-d( Ap Ap Ap Ap Gp Gp Cp Gp Cp Cp Gp Tp Gp Gp Tp C)-

Source:

Thermus aquaticus. Organism_taxid: 271. Gene: pola, pol1. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Synthetic: yes

Resolution:

2.20Å R-factor: 0.173 R-free: 0.224

Authors:

K.Diederichs,A.Marx,K.Betz

Key ref:

K.Betz et al. (2010). Structures of DNA polymerases caught processing size-augmented nucleotide probes. Angew Chem Int Ed Engl, 49, 5181-5184. PubMed id: 20572212

Date:

19-Mar-10 Release date: 07-Jul-10

Protein chain

P19821  (DPO1_THEAQ) -  DNA polymerase I, thermostable from Thermus aquaticus

Seq: 832 a.a.

Struc: 539 a.a.

DNA/RNA chains

G-A-C-C-A-C-G-G-C-G-C-DOC 12 bases

A-A-A-A-G-G-C-G-C-C-G-T-G-G-T-C 16 bases

Enzyme reactions

• Enzyme class: E.C.2.7.7.7 - DNA-directed Dna polymerase.
• Reaction: DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

Angew Chem Int Ed Engl 49:5181-5184 (2010)

PubMed id: 20572212

Structures of DNA polymerases caught processing size-augmented nucleotide probes.

K.Betz, F.Streckenbach, A.Schnur, T.Exner, W.Welte, K.Diederichs, A.Marx.

ABSTRACT

No abstract given.