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PDBsum entry 2wns

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protein ligands metals Protein-protein interface(s) links
Transferase PDB id
2wns
Jmol
Contents
Protein chains
197 a.a. *
Ligands
OMP ×2
Metals
_CL
Waters ×237
* Residue conservation analysis
PDB id:
2wns
Name: Transferase
Title: Human orotate phosphoribosyltransferase (oprtase) domain of uridine 5'-monophosphate synthase (umps) in complex with its substrate orotidine 5'-monophosphate (omp)
Structure: Orotate phosphoribosyltransferase. Chain: a, b. Fragment: oprtase domain, residues 7-203. Synonym: oprtase, uridine 5'-monophosphate synthase. Engineered: yes
Source: Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Resolution:
1.90Å     R-factor:   0.182     R-free:   0.227
Authors: M.Moche,A.Roos,C.H.Arrowsmith,H.Berglund,C.Bountra, R.Collins,A.M.Edwards,S.Flodin,A.Flores,S.Graslund, M.Hammarstrom,A.Johansson,I.Johansson,T.Karlberg, T.Kotyenova,A.Kotzch,T.K.Nielsen,T.Nyman,C.Persson, J.Sagemark,H.Schueler,P.Schutz,M.I.Siponen,L.Svensson, A.G.Thorsell,L.Tresaugues,S.Vandenberg,J.Weigelt,M.Welin, M.Wisniewska,P.Nordlund
Key ref: M.Moche et al. Human orotate phosphoribosyltransferase (oprtase) domain of uridine 5'-Monophosphate synthase (umps) in complex with its substrate orotidine 5'- Monophosphate (omp). To be published, .
Date:
19-Jul-09     Release date:   11-Aug-09    
PROCHECK
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 Headers
 References

Protein chains
Pfam   ArchSchema ?
P11172  (UMPS_HUMAN) -  Uridine 5'-monophosphate synthase
Seq:
Struc:
480 a.a.
197 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class 1: E.C.2.4.2.10  - Orotate phosphoribosyltransferase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]

      Pathway:
Pyrimidine Biosynthesis
      Reaction: Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate
Orotidine 5'-phosphate
Bound ligand (Het Group name = OMP)
corresponds exactly
+ diphosphate
= orotate
+ 5-phospho-alpha-D-ribose 1-diphosphate
   Enzyme class 2: E.C.4.1.1.23  - Orotidine-5'-phosphate decarboxylase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]

      Pathway:
      Reaction: Orotidine 5'-phosphate = UMP + CO2
Orotidine 5'-phosphate
Bound ligand (Het Group name = OMP)
corresponds exactly
= UMP
+ CO(2)
Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     nucleoside metabolic process   2 terms 
  Biochemical function     orotate phosphoribosyltransferase activity     1 term