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PDBsum entry 2w8h
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Membrane protein
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PDB id
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2w8h
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Contents |
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* Residue conservation analysis
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PDB id:
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| Name: |
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Membrane protein
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Title:
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Crystal structure of spin labeled wza24-345.
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Structure:
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Putative outer membrane lipoprotein wza. Chain: a, b, c, d, e, f, g, h. Fragment: periplasmic domain, residues 22-345. Synonym: wza. Engineered: yes. Mutation: yes. Other_details: labeled with mtssl at postion 335
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Source:
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Escherichia coli. Organism_taxid: 562. Expressed in: escherichia coli. Expression_system_taxid: 562.
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Resolution:
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2.76Å
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R-factor:
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0.212
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R-free:
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0.229
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Authors:
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G.Hagelueken,W.J.Ingledew,H.Huang,B.Petrovic-Stojanovska,C.Whitfield, H.Elmkami,O.Schiemann,J.H.Naismith
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Key ref:
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G.Hagelueken
et al.
(2009).
PELDOR spectroscopy distance fingerprinting of the octameric outer-membrane protein Wza from Escherichia coli.
Angew Chem Int Ed Engl,
48,
2904-2906.
PubMed id:
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Date:
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16-Jan-09
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Release date:
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10-Feb-09
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PROCHECK
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Headers
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References
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Q9X4B7
(Q9X4B7_ECOLX) -
Putative outer membrane lipoprotein Wza from Escherichia coli
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Seq:
Struc:
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379 a.a.
322 a.a.*
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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*
PDB and UniProt seqs differ
at 1 residue position (black
cross)
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Angew Chem Int Ed Engl
48:2904-2906
(2009)
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PubMed id:
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PELDOR spectroscopy distance fingerprinting of the octameric outer-membrane protein Wza from Escherichia coli.
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G.Hagelueken,
W.J.Ingledew,
H.Huang,
B.Petrovic-Stojanovska,
C.Whitfield,
H.ElMkami,
O.Schiemann,
J.H.Naismith.
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ABSTRACT
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Distance fingerprinting: Pulsed electron-electron double resonance spectroscopy
(PELDOR) is applied to the octameric membrane protein complex Wza of E. coli.
The data yielded a detailed distance fingerprint of its periplasmic region that
compares favorably to the crystal structure. These results provide the
foundation to study conformation changes from interaction with partner proteins.
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Links
