PDBsum entry 2w3v

Oxidoreductase

PDB id: 2w3v

Contents

Protein chain

165 a.a. *

Ligands

NDP

TOP

Waters ×160

* Residue conservation analysis

PDB id:

2w3v

Name:

Oxidoreductase

Title:

Mycobacterium avium dihydrofolate reductase complexed with NADPH and trimethoprim

Structure:

Dihydrofolate reductase. Chain: a. Fragment: residues 1-167. Engineered: yes

Source:

Mycobacterium avium. Organism_taxid: 1764. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Other_details: strain obtained from the aids research and reference reagent program, division of aids, niaid, nih, catalog number 1786.

Resolution:

1.89Å R-factor: 0.182 R-free: 0.224

Authors:

A.K.W.Leung,R.C.Reynolds,D.W.Borhani

Key ref:

A.K.W.Leung et al. Structural basis for selective inhibition of mycobacterium avium dihydrofolate reductase by a lipophilic antifolate. To be published, .

Date:

16-Nov-08 Release date: 17-Nov-09

Protein chain

O30463  (O30463_MYCAV) -  Dihydrofolate reductase from Mycobacterium avium

Seq: 181 a.a.

Struc: 165 a.a.

Enzyme reactions

• Enzyme class: E.C.1.5.1.3 - dihydrofolate reductase.
• Pathway: Folate Coenzymes
• Reaction: (6S)-5,6,7,8-tetrahydrofolate + NADP⁺ = 7,8-dihydrofolate + NADPH + H⁺

(6S)-5,6,7,8-tetrahydrofolate + NADP(+) (Bound ligand (Het Group name = NDP) corresponds exactly) = 7,8-dihydrofolate + NADPH + H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site