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PDBsum entry 2q6t
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Contents |
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* Residue conservation analysis
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Enzyme class:
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E.C.3.6.4.12
- Dna helicase.
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Reaction:
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ATP + H2O = ADP + phosphate + H+
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ATP
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+
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H2O
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=
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ADP
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+
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phosphate
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+
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H(+)
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Molecule diagrams generated from .mol files obtained from the
KEGG ftp site
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Nucleic Acids Res
35:4728-4736
(2007)
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PubMed id:
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The crystal structure of the Thermus aquaticus DnaB helicase monomer.
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S.Bailey,
W.K.Eliason,
T.A.Steitz.
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ABSTRACT
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The ring-shaped hexameric DnaB helicase unwinds duplex DNA at the replication
fork of eubacteria. We have solved the crystal structure of the full-length
Thermus aquaticus DnaB monomer, or possibly dimer, at 2.9 A resolution. DnaB is
a highly flexible two domain protein. The C-terminal domain exhibits a RecA-like
core fold and contains all the conserved sequence motifs that are characteristic
of the DnaB helicase family. The N-terminal domain contains an additional
helical hairpin that makes it larger than previously appreciated. Several DnaB
mutations that modulate its interaction with primase are found in this hairpin.
The similarity in the fold of the DnaB N-terminal domain with that of the
C-terminal helicase-binding domain (HBD) of the DnaG primase also includes this
hairpin. Comparison of hexameric homology models of DnaB with the structure of
the papillomavirus E1 helicase suggests the two helicases may function through
different mechanisms despite their sharing a common ancestor.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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M.Makowska-Grzyska,
and
J.M.Kaguni
(2010).
Primase directs the release of DnaC from DnaB.
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Mol Cell,
37,
90.
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T.Kashav,
R.Nitharwal,
S.A.Abdulrehman,
A.Gabdoulkhakov,
W.Saenger,
S.K.Dhar,
and
S.Gourinath
(2009).
Three-dimensional structure of N-terminal domain of DnaB helicase and helicase-primase interactions in Helicobacter pylori.
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PLoS One,
4,
e7515.
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PDB code:
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Y.H.Lo,
K.L.Tsai,
Y.J.Sun,
W.T.Chen,
C.Y.Huang,
and
C.D.Hsiao
(2009).
The crystal structure of a replicative hexameric helicase DnaC and its complex with single-stranded DNA.
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Nucleic Acids Res,
37,
804-814.
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PDB codes:
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Y.Xie,
and
Z.G.He
(2009).
Characterization of Physical Interaction between Replication Initiator Protein DnaA and Replicative Helicase from Mycobacterium tuberculosis H37Rv.
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Biochemistry (Mosc),
74,
1320-1327.
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E.J.Enemark,
and
L.Joshua-Tor
(2008).
On helicases and other motor proteins.
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Curr Opin Struct Biol,
18,
243-257.
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T.Biswas,
and
O.V.Tsodikov
(2008).
Hexameric ring structure of the N-terminal domain of Mycobacterium tuberculosis DnaB helicase.
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FEBS J,
275,
3064-3071.
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PDB code:
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Y.Matsushima,
C.L.Farr,
L.Fan,
and
L.S.Kaguni
(2008).
Physiological and biochemical defects in carboxyl-terminal mutants of mitochondrial DNA helicase.
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J Biol Chem,
283,
23964-23971.
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S.Bailey,
W.K.Eliason,
and
T.A.Steitz
(2007).
Structure of hexameric DnaB helicase and its complex with a domain of DnaG primase.
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Science,
318,
459-463.
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PDB codes:
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
code is
shown on the right.
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Links
