PDBsum entry 1wcf

Hydrolase

PDB id: 1wcf

Contents

Protein chain

162 a.a. *

Ligands

PO4

Metals

__K ×2

Waters ×279

* Residue conservation analysis

PDB id:

1wcf

Name:

Hydrolase

Title:

1.54 a crystal structure of rv3628, mycobacterium tuberculosis inorganic pyrophosphatase (ppase) at ph7.0

Structure:

Inorganic pyrophosphatase. Chain: a. Synonym: pyrophosphate phospho-hydrolase, ppase. Engineered: yes

Source:

Mycobacterium tuberculosis. Organism_taxid: 83332. Strain: h37rv. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Biol. unit:

Hexamer (from PDB file)

Resolution:

1.54Å R-factor: 0.157 R-free: 0.170

Authors:

S.Benini,K.S.Wilson

Key ref:

S.Benini and K.Wilson (2011). Structure of the Mycobacterium tuberculosis soluble inorganic pyrophosphatase Rv3628 at pH 7.0. Acta Crystallogr Sect F Struct Biol Cryst Commun, 67, 866-870. PubMed id: 21821883 DOI: 10.1107/S1744309111023323

Date:

13-Nov-04 Release date: 12-Jul-06

Protein chain

P9WI55  (IPYR_MYCTU) -  Inorganic pyrophosphatase from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

Seq: 162 a.a.

Struc: 162 a.a.

Enzyme reactions

• Enzyme class: E.C.3.6.1.1 - inorganic diphosphatase.
• Reaction: diphosphate + H2O = 2 phosphate + H⁺

diphosphate + H2O = 2 × phosphate + H(+) (Bound ligand (Het Group name = PO4) corresponds exactly)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

DOI no: 10.1107/S1744309111023323

Acta Crystallogr Sect F Struct Biol Cryst Commun 67:866-870 (2011)

PubMed id: 21821883

Structure of the Mycobacterium tuberculosis soluble inorganic pyrophosphatase Rv3628 at pH 7.0.

S.Benini, K.Wilson.

ABSTRACT

No abstract given.