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PDBsum entry 1rtb
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Hydrolase(nucleic acid,RNA)
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PDB id
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1rtb
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Contents |
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* Residue conservation analysis
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Enzyme class:
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E.C.4.6.1.18
- pancreatic ribonuclease.
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Reaction:
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1.
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an [RNA] containing cytidine + H2O = an [RNA]-3'-cytidine- 3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA]
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2.
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an [RNA] containing uridine + H2O = an [RNA]-3'-uridine-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA]
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J Biol Chem
267:22230-22236
(1992)
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PubMed id:
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Crystal structure disposition of thymidylic acid tetramer in complex with ribonuclease A.
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D.L.Birdsall,
A.McPherson.
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ABSTRACT
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The crystal structure of ribonuclease A with bound thymidylic acid tetramer is
reported at 2.5-A resolution. The diffusion of the tetramer into native
orthorhombic crystals of the ribonuclease allows for the formation of a
structurally stable complex where the single-stranded nucleic acid enters and
leaves the enzyme's catalytic region in a persistent 5'-3' direction. The
binding of the tetramer to the enzyme's surface is facilitated and mediated by
electrostatic interactions between basic protein residues and nucleotide
phosphates. Two pyrimidine nucleotides are bound to the enzyme's active site in
a manner similar to that observed for other complexes between ribonuclease A and
nucleic acid oligomers.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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S.B.Larson,
J.S.Day,
C.Nguyen,
R.Cudney,
and
A.McPherson
(2010).
Structure of bovine pancreatic ribonuclease complexed with uridine 5'-monophosphate at 1.60 A resolution.
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Acta Crystallogr Sect F Struct Biol Cryst Commun,
66,
113-120.
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PDB code:
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S.Ganguli,
K.Yoshimoto,
S.Tomita,
H.Sakuma,
T.Matsuoka,
K.Shiraki,
and
Y.Nagasaki
(2010).
Improving the heat resistance of ribonuclease A by the addition of poly(N,N-diethylaminoethyl methacrylate)-graft-poly(ethylene glycol) (PEAMA-g-PEG).
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Macromol Biosci,
10,
853-859.
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N.G.Beloglazova,
M.M.Fabani,
M.A.Zenkova,
E.V.Bichenkova,
N.N.Polushin,
V.V.Sil'nikov,
K.T.Douglas,
and
V.V.Vlassov
(2004).
Sequence-specific artificial ribonucleases. I. Bis-imidazole-containing oligonucleotide conjugates prepared using precursor-based strategy.
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Nucleic Acids Res,
32,
3887-3897.
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Y.Liu,
G.Gotte,
M.Libonati,
and
D.Eisenberg
(2002).
Structures of the two 3D domain-swapped RNase A trimers.
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Protein Sci,
11,
371-380.
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PDB code:
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Z.Morávek,
S.Neidle,
and
B.Schneider
(2002).
Protein and drug interactions in the minor groove of DNA.
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Nucleic Acids Res,
30,
1182-1191.
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Y.Liu,
P.J.Hart,
M.P.Schlunegger,
and
D.Eisenberg
(1998).
The crystal structure of a 3D domain-swapped dimer of RNase A at a 2.1-A resolution.
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Proc Natl Acad Sci U S A,
95,
3437-3442.
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PDB code:
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
code is
shown on the right.
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Links
