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PDBsum entry 1rtb
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Hydrolase(nucleic acid,RNA)
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PDB id
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1rtb
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References listed in PDB file
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Key reference
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Title
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Crystal structure disposition of thymidylic acid tetramer in complex with ribonuclease a.
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Authors
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D.L.Birdsall,
A.Mcpherson.
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Ref.
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J Biol Chem, 1992,
267,
22230-22236.
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PubMed id
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Abstract
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The crystal structure of ribonuclease A with bound thymidylic acid tetramer is
reported at 2.5-A resolution. The diffusion of the tetramer into native
orthorhombic crystals of the ribonuclease allows for the formation of a
structurally stable complex where the single-stranded nucleic acid enters and
leaves the enzyme's catalytic region in a persistent 5'-3' direction. The
binding of the tetramer to the enzyme's surface is facilitated and mediated by
electrostatic interactions between basic protein residues and nucleotide
phosphates. Two pyrimidine nucleotides are bound to the enzyme's active site in
a manner similar to that observed for other complexes between ribonuclease A and
nucleic acid oligomers.
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Secondary reference #1
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Title
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Comparison of two independently refined models of ribonuclease-A
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Authors
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A.Wlodawer,
N.Borkakoti,
D.S.Moss,
B.Howlin.
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Ref.
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acta crystallogr ,sect b, 1986,
42,
379.
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Secondary reference #2
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Title
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Nuclear magnetic resonance and neutron diffraction studies of the complex of ribonuclease a with uridine vanadate, A transition-State analogue.
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Authors
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B.Borah,
C.W.Chen,
W.Egan,
M.Miller,
A.Wlodawer,
J.S.Cohen.
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Ref.
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Biochemistry, 1985,
24,
2058-2067.
[DOI no: ]
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PubMed id
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Secondary reference #3
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Title
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Active site of rnase: neutron diffraction study of a complex with uridine vanadate, A transition-State analog.
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Authors
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A.Wlodawer,
M.Miller,
L.Sjölin.
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Ref.
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Proc Natl Acad Sci U S A, 1983,
80,
3628-3631.
[DOI no: ]
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PubMed id
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Secondary reference #4
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Title
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Structure of ribonuclease a: results of joint neutron and X-Ray refinement at 2.0-A resolution.
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Authors
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A.Wlodawer,
L.Sjölin.
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Ref.
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Biochemistry, 1983,
22,
2720-2728.
[DOI no: ]
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PubMed id
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Secondary reference #5
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Title
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The refined crystal structure of ribonuclease a at 2.0 a resolution.
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Authors
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A.Wlodawer,
R.Bott,
L.Sjölin.
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Ref.
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J Biol Chem, 1982,
257,
1325-1332.
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PubMed id
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Secondary reference #6
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Title
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Hydrogen exchange in rnase a: neutron diffraction study.
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Authors
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A.Wlodawer,
L.Sjölin.
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Ref.
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Proc Natl Acad Sci U S A, 1982,
79,
1418-1422.
[DOI no: ]
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PubMed id
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Secondary reference #7
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Title
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Structure of ribonuclease a: X-Ray and neutron refinement
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Authors
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A.Wlodawer,
R.Bott,
L.Sjolin.
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Ref.
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acta crystallogr ,sect a, 1981,
37,
13.
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Secondary reference #8
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Title
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Joint refinement of macromolecular structures with X-Ray and neutron single-Crystal diffraction data
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Authors
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A.Wlodawer,
W.A.Hendrickson.
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Ref.
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acta crystallogr ,sect a, 1981,
37,
8.
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Secondary reference #9
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Title
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Orientation of histidine residues in rnase a: neutron diffraction study.
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Authors
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A.Wlodawer,
L.Sjölin.
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Ref.
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Proc Natl Acad Sci U S A, 1981,
78,
2853-2855.
[DOI no: ]
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PubMed id
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Secondary reference #10
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Title
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Studies of ribonuclease-A by X-Ray and neutron diffraction
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Author
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A.Wlodawer.
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Ref.
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acta crystallogr ,sect b, 1980,
36,
1826.
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