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PDBsum entry 3jw1

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protein ligands Protein-protein interface(s) links
Hydrolase PDB id
3jw1
Jmol
Contents
Protein chains
124 a.a. *
Ligands
U5P ×2
Waters ×223
* Residue conservation analysis
PDB id:
3jw1
Name: Hydrolase
Title: Crystal structure of bovine pancreatic ribonuclease complexe uridine-5'-monophosphate at 1.60 a resolution
Structure: Ribonuclease pancreatic. Chain: a, b. Synonym: rnase 1, rnase a. Ec: 3.1.27.5
Source: Bos taurus. Bovine,cow,domestic cattle,domestic cow. Organism_taxid: 9913
Resolution:
1.60Å     R-factor:   0.203     R-free:   0.253
Authors: S.B.Larson,J.S.Day,C.Nguyen,R.Cudney,A.Mcpherson,Center For Throughput Structural Biology (Chtsb)
Key ref: S.B.Larson et al. (2010). Structure of bovine pancreatic ribonuclease complexed with uridine 5'-monophosphate at 1.60 A resolution. Acta Crystallogr Sect F Struct Biol Cryst Commun, 66, 113-120. PubMed id: 20124705
Date:
17-Sep-09     Release date:   06-Oct-09    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
P61823  (RNAS1_BOVIN) -  Ribonuclease pancreatic
Seq:
Struc:
150 a.a.
124 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class: E.C.3.1.27.5  - Pancreatic ribonuclease.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in C-P or U-P with 2',3'-cyclic phosphate intermediates.
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     extracellular region   1 term 
  Biological process     metabolic process   3 terms 
  Biochemical function     nucleic acid binding     7 terms  

 

 
Acta Crystallogr Sect F Struct Biol Cryst Commun 66:113-120 (2010)
PubMed id: 20124705  
 
 
Structure of bovine pancreatic ribonuclease complexed with uridine 5'-monophosphate at 1.60 A resolution.
S.B.Larson, J.S.Day, C.Nguyen, R.Cudney, A.McPherson.
 
  ABSTRACT  
 
Bovine pancreatic ribonuclease A (RNase A) was crystallized from a mixture of small molecules containing basic fuchsin, tobramycin and uridine 5'-monophosphate (U5P). Solution of the crystal structure revealed that the enzyme was selectively bound to U5P, with the pyrimidine ring of U5P residing in the pyrimidine-binding site at Thr45. The structure was refined to an R factor of 0.197 and an R(free) of 0.253.