- Course overview
- Search within this course
- What is UniProt?
- Where does the data come from?
- Why do we need UniProt?
- When to use UniProt
- Quiz: Check your learning I
- How to access and navigate UniProt
- How to search UniProt
- Annotation score
- Quiz: Check your learning II
- Exploring a UniProtKB entry
- How to get data from UniProt
- How to submit data to UniProt
- When to use UniProt: guided examples
- Exercise: finding entries with 3D structures
- Exercise: mapping other database identifiers to UniProt
- Summary
- Get help and support on UniProt
- References
- Next steps
- Your feedback
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Aligning multiple protein sequences
A sequence alignment is a way of arranging the primary sequences of a protein to identify regions of similarity that may be a consequence of functional, structural, or evolutionary relationships between the sequences.
- Click on the Align link in the header bar to align two or more protein sequences with the Clustal Omega program
- Enter either protein sequences in FASTA format or UniProt identifiers into the form field
- Click the ‘Run Align’ button
- Advanced users can change the default alignment parameters
The following kinds of UniProt identifiers are supported:
| P00750 | UniProtKB entry |
|---|---|
| P00750-2 | UniProtKB entry isoform sequence |
| A4_HUMAN | UniProtKB entry name |
| UPI0000000001 | UniParc entry |
Each UniProtKB entry which contains both a sequence and one or more isoforms of that sequence enables you to align the canonical sequence and its isoforms. All relevant results pages (such as UniProtKB, UniRef, UniParc and tool results) provide an ‘Align’ button to run alignments directly by selecting entries with checkboxes. You can also run alignments from within the Basket.
 You can learn more about sequence alignments on the UniProt help page. |