- Course overview
- Search within this course
- What is the IMPC?
- Translating to other species and learning about human disease
- The home page of the IMPC website
- Searching for a gene of interest
- Gene pages
- Chart pages
- Searching for phenotypes
- Phenotype pages
- Late Adult data
- Image data
- Data downloads
- Try it yourself
- Quiz: check your learning
- Summary
- Your feedback
- Get help and support on the IMPC
- References
All materials are free cultural works licensed under a Creative Commons Attribution 4.0 International (CC BY 4.0) license, except where further licensing details are provided.
Mouse production
The IMPC produces single-gene knockouts. To knockout genes, the IMPC is using CRISPR/Cas9 technology, a gene editing technique.
Prior to this, the gene knockout technology used by the IMPC was based on embryonic stem (ES) cells.
These strategies rely on the identification of a ‘critical’ exon common to all transcript variants that, when deleted, disrupts gene function.
You can find more information about Mouse Production in our Documentation pages.
In this video, IMPC researcher Lauryl Nutter talks about how CRISPR/Cas9 is being used by the IMPC