Long-read sequencing

Variant calling

Trainers: Mohab Helmy

Overview: Recent advances in sequencing technologies made it feasible to obtain significantly longer reads that can be used to produce contagious genome assemblies. In this session, we will cover how long-reads can be utilised to assemble high-quality genomes as well as to provide novel insights into complex areas of genomes including long repeats and complex structural variants.

Learning outcomes:

By the end of this session you will be able to:

• Describe the advantages and disadvantages of using long-read sequencing vs. short-read sequencing

Materials:

• ‘Long-read sequencing’ slides