Genome bioinformatics: from short- to long-read sequencing, which ran in November 2025, provided a guide to the technology, analysis workflows, tools, and resources for next generation sequencing data analysis.

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Using these materials

These course materials provide a mixture of presentations and practicals to help advance your knowledge and skills in the analysis of biological data. You may select your topic of interest from the Course content page to view the relevant materials or work your way through all the course materials. 

To find out more about the trainers who created these materials, follow the links from the Course content page or go directly to the Trainer biographies page.

In the Further learning section you may explore the details about the EMBL-EBI’s free access online tutorials and webinars on a variety of life sciences topics.

If you would like to provide feedback on this set of course materials, please use the form on the Your feedback page.

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Learning outcomes

After the course participants will be able to:

• State the advantages and limitations of short- and long-read sequencing technologies
• Apply appropriate QC and aligners to unassembled short- and long-reads
• Perform variant calling analysis and annotation
• Create, scale-up, and automate genomics pipelines
• Access genomic datasets from online public resources

Material collection editors

• Chiara Batini, University of Leicester
• Pille Hallast, The Jackson Laboratory for Genomic Medicine
• Daria Sokolova, EMBL-EBI
• Maxime Tarabichi, Institut de Recherche Interdisciplinaire en Biologie Humaine et Moléculaire (IRIBHM)

DOI: 10.6019/TOL.GenomeBioinformatics-t.2023.00001.1