Introduction to ChIP-seq Data Analysis and Visualisation using Ensembl
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Date:
Tuesday 22 - Wednesday 23 November 2016Venue:
University of Edinburgh - The University of Edinburgh, Old College, Edinburgh, EH8 9YL, United KingdomApplication deadline:
Tuesday 25 October 2016Participation:
Open application with selectionRegistration fee:
£310Registration closed
Dates additional information:
Registration for this course is being administered by Bert Overduin. Please contact Bert for further information about this course.Course Overview
ChIP-seq (Chromatin ImmunoPrecipitation followed by Sequencing) is a popular high-throughput sequencing assay to identify binding sites of DNA-associated proteins and histone modifications. Determining how proteins interact with DNA and the epigenetic landscape is essential for elucidating the regulation of gene expression. The aim of this workshop is to familiarise the participants with the primary analysis of ChIP-seq data sets by providing a balanced set of lectures and practicals on analysis methodologies. Practicals include publicly available ChIP-seq datasets, processed using widely used and open-source software programs (e.g. FASTQC, BWA, samtools, bedtools, wiggletools, MACS2, MEME, TOMTOM, ngsplot) and visualised on the Ensembl genome browser.
Audience
Graduates, postgraduates, and PIs, who are using, or planning to use, ChIP-seq technology in their research and want to learn how to process and analyse ChIP-seq data.
Learning outcomes
- Learn about ChIP-seq data analysis using a variety of methodologies
- Get hands-on experience using open-source software programs to perform ChIP-seq analysis
- Learn how to visualise ChIP-seq data using the Ensembl genome browser