PDBsum entry 9lrc

Membrane protein

PDB id: 9lrc

Contents

Protein chain

282 a.a.

Ligands

HSM

Waters ×1

PDB id:

9lrc

Name:

Membrane protein

Title:

Cryo-em structure of the histamine h4 receptor-gi protein complex (receptor focused)

Structure:

Histamine h4 receptor,genome polyprotein. Chain: r. Synonym: h4r,hh4r,axor35,g-protein coupled receptor 105,gprv53,pfi- 013,sp9144. Ec: 3.4.22.29,3.6.1.15,3.4.22.28,2.7.7.48. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: hrh4, gpcr105. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108

Authors:

Y.Matsuzaki,F.K.Sano,H.S.Oshima,H.Akasaka,K.Kobayashi,T.Tanaka, Y.Itoh,W.Shihoya,Y.Kise,T.Kusakizako,O.Nureki

Key ref:

Y.Matsuzaki et al. Structural insights into ligand recognition and g pro preferences across histamine receptors. To be published, . PubMed id: 40579541

Date:

30-Jan-25 Release date: 11-Jun-25

Protein chain

B6F2F5  (B6F2F5_HE71) -  Genome polyprotein from Human enterovirus 71

Seq: 2438 a.a.

Struc: 282 a.a.*

Protein chain

Q9H3N8  (HRH4_HUMAN) -  Histamine H4 receptor from Homo sapiens

Seq: 390 a.a.

Struc: 282 a.a.

* PDB and UniProt seqs differ at 255 residue positions (black crosses)

Enzyme reactions

• Enzyme class 1: E.C.2.7.7.48 - RNA-directed Rna polymerase.
• Reaction: RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate
• Enzyme class 2: E.C.3.4.22.28 - picornain 3C.
• Reaction: Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
• Enzyme class 3: E.C.3.4.22.29 - picornain 2A.
• Reaction: Selective cleavage of Tyr-|-Gly bond in the picornavirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
• Enzyme class 4: E.C.3.6.1.15 - nucleoside-triphosphate phosphatase.
• Reaction: a ribonucleoside 5'-triphosphate + H2O = a ribonucleoside 5'-diphosphate + phosphate + H⁺

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site