PDBsum entry 9gyo

Membrane protein

PDB id: 9gyo

Contents

Protein chains

231 a.a.

339 a.a.

58 a.a.

127 a.a.

273 a.a.

Ligands

FWX

CLR ×3

PDB id:

9gyo

Name:

Membrane protein

Title:

Cryoem structure of gs-coupled gpbar with small molecule agonist p395

Structure:

Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short. Chain: a. Synonym: adenylate cyclase-stimulating g alpha protein. Engineered: yes. Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1. Chain: b. Engineered: yes.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: gnas, gnas1, gsp. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Gene: gnb1. Gene: gng2. Lama glama.

Authors:

A.Frechard,I.Brooks

Key ref:

J.Castel et al. Deciphering molecular determinants of gpcr-G protein receptor interactions by complementary integrative structural biology methods. To be published, . PubMed id: 40858717

Date:

02-Oct-24 Release date: 09-Oct-24

Protein chain

P63092  (GNAS2_HUMAN) -  Guanine nucleotide-binding protein G(s) subunit alpha isoforms short from Homo sapiens

Seq: 394 a.a.

Struc: 231 a.a.*

Protein chain

P62873  (GBB1_HUMAN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 from Homo sapiens

Seq: 340 a.a.

Struc: 339 a.a.

Protein chain

P59768  (GBG2_HUMAN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 from Homo sapiens

Seq: 71 a.a.

Struc: 58 a.a.

Protein chain

No UniProt id for this chain

Struc: 127 a.a.

Protein chain

P0ABE7  (C562_ECOLX) -  Soluble cytochrome b562 from Escherichia coli

Seq: 128 a.a.

Struc: 273 a.a.*

Protein chain

Q8TDU6  (GPBAR_HUMAN) -  G-protein coupled bile acid receptor 1 from Homo sapiens

Seq: 330 a.a.

Struc: 273 a.a.

* PDB and UniProt seqs differ at 74 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chain A: E.C.3.6.5.- - ?????