PDBsum entry 9guj

DNA binding protein

PDB id: 9guj

Contents

Protein chains

213 a.a.

84 a.a.

85 a.a.

DNA/RNA

Ligands

AKG ×4

PDB id:

9guj

Name:

DNA binding protein

Title:

Crystal structure of transcription factor ntca from synechococcus elongatus in complex with its transcriptional co- activator pipx and its target DNA (crystal ii)

Structure:

Global nitrogen regulator. Chain: d, f, a, b. Engineered: yes. DNA. Chain: g, i, c, e. Engineered: yes. Pipx. Chain: j, h, k. Engineered: yes

Source:

Synechococcus elongatus pcc 7942 = fachb-805. Organism_taxid: 1140. Gene: ntca, synpcc7942_0127. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Expression_system_variant: rosetta. Synthetic: yes. Gene: synpcc7942_2061.

Resolution:

4.30Å R-factor: 0.285 R-free: 0.316

Authors:

J.L.Llacer,A.Forcada-Nadal,V.Rubio

Key ref:

A.Forcada-Nadal et al. (2025). Structures of the cyanobacterial nitrogen regulators and pipx complexed to DNA shed light on DNA binding b and implicate pipx in the recruitment of RNA polymera. Nucleic acids res., 53, . PubMed id: 39995039 DOI: 10.1093/NAR/GKAF096

Date:

19-Sep-24 Release date: 12-Mar-25

Protein chains

P29283  (NTCA_SYNE7) -  Global nitrogen regulator from Synechococcus elongatus (strain ATCC 33912 / PCC 7942 / FACHB-805)

Seq: 222 a.a.

Struc: 213 a.a.

Protein chains

Q7X386  (Q7X386_SYNE7) -  PipX from Synechococcus elongatus (strain ATCC 33912 / PCC 7942 / FACHB-805)

Seq: 89 a.a.

Struc: 84 a.a.

Protein chain

Q7X386  (Q7X386_SYNE7) -  PipX from Synechococcus elongatus (strain ATCC 33912 / PCC 7942 / FACHB-805)

Seq: 89 a.a.

Struc: 85 a.a.

DNA/RNA chains

A-T-T-T-T-T-A-T-G-T-A-T-C-A-G-C-T-G-A-T-A-C-A-T-A-A-A-A-A-T 30 bases

A-T-T-T-T-T-A-T-G-T-A-T-C-A-G-C-T-G-A-T-A-C-A-T-A-A-A-A-A-T 30 bases

T-T-T-T-A-T-G-T-A-T-C-A-G-C-T-G-A-T-A-C-A-T-A-A-A-A-A-T 28 bases

A-T-T-T-T-T-A-T-G-T-A-T-C-A-G-C-T-G-A-T-A-C-A-T-A-A-A-A-A-T 30 bases