PDBsum entry 9lwp

Membrane protein

PDB id: 9lwp

Contents

Protein chains

271 a.a.

211 a.a.

338 a.a.

56 a.a.

PDB id:

9lwp

Name:

Membrane protein

Title:

Cryo-em structure of the unliganded human brs3-gq complex

Structure:

Bombesin receptor subtype-3,oplophorus-luciferin 2- monooxygenase catalytic subunit. Chain: r. Synonym: brs-3,19kolase. Engineered: yes. Guanine nucleotide-binding protein g(i) subunit alpha-2, guanine nucleotide-binding protein g(s) subunit alpha isoforms short. Chain: a. Synonym: adenylate cyclase-inhibiting g alpha protein,adenylate

Source:

Homo sapiens, oplophorus gracilirostris. Human. Organism_taxid: 9606, 727944. Gene: brs3. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Homo sapiens. Organism_taxid: 9606. Gene: gnai2, gnai2b, gnas, gnas1, gsp.

Authors:

J.Li,C.Li,Q.Zhou,W.Han,M.Fang,Y.Xu,Y.Mai,J.Cui,H.Xu,Y.Zhang,M.Wang

Key ref:

J.Li et al. Divergent activation patterns of brs3 revealed by two chinese herb-Derived agonists. Acta pharm sin b, . PubMed id: 41132856

Date:

16-Feb-25 Release date: 16-Jul-25

Protein chain

P32247  (BRS3_HUMAN) -  Bombesin receptor subtype-3 from Homo sapiens

Seq: 399 a.a.

Struc: 271 a.a.

Protein chain

Q9GV45  (LUCI_OPLGR) -  Oplophorus-luciferin 2-monooxygenase catalytic subunit from Oplophorus gracilirostris

Seq: 196 a.a.

Struc: 271 a.a.*

Protein chain

P04899  (GNAI2_HUMAN) -  Guanine nucleotide-binding protein G(i) subunit alpha-2 from Homo sapiens

Seq: 355 a.a.

Struc: 211 a.a.*

Protein chain

P63092  (GNAS2_HUMAN) -  Guanine nucleotide-binding protein G(s) subunit alpha isoforms short from Homo sapiens

Seq: 394 a.a.

Struc: 211 a.a.*

Protein chain

P54311  (GBB1_RAT) -  Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 from Rattus norvegicus

Seq: 340 a.a.

Struc: 338 a.a.

Protein chain

P63212  (GBG2_BOVIN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 from Bos taurus

Seq: 71 a.a.

Struc: 56 a.a.

* PDB and UniProt seqs differ at 312 residue positions (black crosses)

Enzyme reactions

• Enzyme class 2: Chain A: E.C.3.6.5.- - ?????
• Enzyme class 3: Chain R: E.C.1.13.12.13 - Oplophorus-luciferin 2-monooxygenase.

Pathway:

• Reaction: coelenterazine + O2 = coelenteramide + hnu + CO2

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site