UniProt functional annotation for Q9GV45



	UniProt functional annotation for Q9GV45

UniProt code: Q9GV45.

Organism: Oplophorus gracilirostris (Luminous shrimp).

Taxonomy: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Crustacea; Multicrustacea; Malacostraca; Eumalacostraca; Eucarida; Decapoda; Pleocyemata; Caridea; Oplophoroidea; Oplophoridae; Oplophorus.

Function: Catalytic subunit of oplophorus-luciferin 2-monooxygenase. Oxidoreductase that converts coelenterazine (the oplophorus luciferin) to coelenteramide under emission of blue light with a maximum at 454 nm. Is also active with bisdeoxycoelenterazine. {ECO:0000269|PubMed:10984608}.

Catalytic activity: Reaction=coelenterazine + O2 = CO2 + coelenteramide + hnu; Xref=Rhea:RHEA:20417, ChEBI:CHEBI:2311, ChEBI:CHEBI:15379, ChEBI:CHEBI:16526, ChEBI:CHEBI:30212, ChEBI:CHEBI:41487; EC=1.13.12.13; Evidence={ECO:0000269|PubMed:10984608, ECO:0000269|PubMed:17900925, ECO:0000269|PubMed:629957};

Activity regulation: Inhibited by micromolar Cu(2+). {ECO:0000269|PubMed:17900925}.

Biophysicochemical properties: Kinetic parameters: KM=3.73 uM for coelenterazine {ECO:0000269|PubMed:17900925, ECO:0000269|PubMed:629957}; KM=1.3 uM for bis-coelenterazine {ECO:0000269|PubMed:17900925, ECO:0000269|PubMed:629957}; pH dependence: Optimum pH is 8-9. {ECO:0000269|PubMed:17900925, ECO:0000269|PubMed:629957}; Temperature dependence: Optimum temperature is 40 degrees Celsius. {ECO:0000269|PubMed:17900925, ECO:0000269|PubMed:629957};

Subunit: Heterotetramer of a catalytic 19 kDa and a non-catalytic 35 kDa subunit. {ECO:0000269|PubMed:10984608}.

Subcellular location: Secreted {ECO:0000305}.

Miscellaneous: The shrimp has luminous glands at the base of its antennae and legs, and ejects a cloud of brightly luminescent secretion from the base of its antennae upon stimulation.

Annotations taken from UniProtKB at the EBI.


Organism:		Oplophorus gracilirostris (Luminous shrimp).
Taxonomy:		Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Crustacea; Multicrustacea; Malacostraca; Eumalacostraca; Eucarida; Decapoda; Pleocyemata; Caridea; Oplophoroidea; Oplophoridae; Oplophorus.



Function:		Catalytic subunit of oplophorus-luciferin 2-monooxygenase. Oxidoreductase that converts coelenterazine (the oplophorus luciferin) to coelenteramide under emission of blue light with a maximum at 454 nm. Is also active with bisdeoxycoelenterazine. {ECO:0000269\|PubMed:10984608}.


Catalytic activity:		Reaction=coelenterazine + O2 = CO2 + coelenteramide + hnu; Xref=Rhea:RHEA:20417, ChEBI:CHEBI:2311, ChEBI:CHEBI:15379, ChEBI:CHEBI:16526, ChEBI:CHEBI:30212, ChEBI:CHEBI:41487; EC=1.13.12.13; Evidence={ECO:0000269\|PubMed:10984608, ECO:0000269\|PubMed:17900925, ECO:0000269\|PubMed:629957};
Activity regulation:		Inhibited by micromolar Cu(2+). {ECO:0000269\|PubMed:17900925}.
Biophysicochemical properties:		Kinetic parameters: KM=3.73 uM for coelenterazine {ECO:0000269\|PubMed:17900925, ECO:0000269\|PubMed:629957}; KM=1.3 uM for bis-coelenterazine {ECO:0000269\|PubMed:17900925, ECO:0000269\|PubMed:629957}; pH dependence: Optimum pH is 8-9. {ECO:0000269\|PubMed:17900925, ECO:0000269\|PubMed:629957}; Temperature dependence: Optimum temperature is 40 degrees Celsius. {ECO:0000269\|PubMed:17900925, ECO:0000269\|PubMed:629957};
Subunit:		Heterotetramer of a catalytic 19 kDa and a non-catalytic 35 kDa subunit. {ECO:0000269\|PubMed:10984608}.
Subcellular location:		Secreted {ECO:0000305}.
Miscellaneous:		The shrimp has luminous glands at the base of its antennae and legs, and ejects a cloud of brightly luminescent secretion from the base of its antennae upon stimulation.