PDBsum entry 9lre

Membrane protein

PDB id: 9lre

Contents

Protein chains

209 a.a.

338 a.a.

232 a.a.

57 a.a.

282 a.a.

Ligands

HSM

Waters ×1

PDB id:

9lre

Name:

Membrane protein

Title:

Cryo-em structure of the histamine h4 receptor-gi protein complex (overall)

Structure:

Guanine nucleotide-binding protein g(i) subunit alpha-1. Chain: a. Synonym: adenylate cyclase-inhibiting g alpha protein. Engineered: yes. Mutation: yes. Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1. Chain: b. Synonym: transducin beta chain 1.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: gnai1. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Rattus norvegicus. Norway rat. Organism_taxid: 10116.

Authors:

Y.Matsuzaki,F.K.Sano,H.S.Oshima,H.Akasaka,K.Kobayashi,T.Tanaka, Y.Itoh,W.Shihoya,Y.Kise,T.Kusakizako,O.Nureki

Key ref:

Y.Matsuzaki et al. Structural insights into ligand recognition and g pro preferences across histamine receptor. To be published, . PubMed id: 40579541

Date:

30-Jan-25 Release date: 11-Jun-25

Protein chain

P63096  (GNAI1_HUMAN) -  Guanine nucleotide-binding protein G(i) subunit alpha-1 from Homo sapiens

Seq: 354 a.a.

Struc: 209 a.a.*

Protein chain

P54311  (GBB1_RAT) -  Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 from Rattus norvegicus

Seq: 340 a.a.

Struc: 338 a.a.

Protein chain

No UniProt id for this chain

Struc: 232 a.a.

Protein chain

P63212  (GBG2_BOVIN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 from Bos taurus

Seq: 71 a.a.

Struc: 57 a.a.

Protein chain

B6F2F5  (B6F2F5_HE71) -  Genome polyprotein from Human enterovirus 71

Seq: 2438 a.a.

Struc: 282 a.a.*

Protein chain

Q9H3N8  (HRH4_HUMAN) -  Histamine H4 receptor from Homo sapiens

Seq: 390 a.a.

Struc: 282 a.a.

* PDB and UniProt seqs differ at 298 residue positions (black crosses)

Enzyme reactions

• Enzyme class 1: Chain A: E.C.3.6.5.- - ?????
• Enzyme class 2: Chain R: E.C.2.7.7.48 - RNA-directed Rna polymerase.
• Reaction: RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate
• Enzyme class 3: Chain R: E.C.3.4.22.28 - picornain 3C.
• Reaction: Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
• Enzyme class 4: Chain R: E.C.3.4.22.29 - picornain 2A.
• Reaction: Selective cleavage of Tyr-|-Gly bond in the picornavirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
• Enzyme class 5: Chain R: E.C.3.6.1.15 - nucleoside-triphosphate phosphatase.
• Reaction: a ribonucleoside 5'-triphosphate + H2O = a ribonucleoside 5'-diphosphate + phosphate + H⁺

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site