PDBsum entry 9lrb

Membrane protein

PDB id: 9lrb

Contents

Protein chains

231 a.a.

340 a.a.

52 a.a.

128 a.a.

267 a.a.

Ligands

HSM

PDB id:

9lrb

Name:

Membrane protein

Title:

Cryo-em structure of the histamine h1 receptor-gs protein complex

Structure:

Guanine nucleotide-binding protein g(s) subunit alpha isoforms short. Chain: a. Synonym: adenylate cyclase-stimulating g alpha protein. Engineered: yes. Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1. Chain: b. Synonym: transducin beta chain 1.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: gnas, gnas1, gsp. Expressed in: escherichia coli. Expression_system_taxid: 562. Rattus norvegicus. Norway rat. Organism_taxid: 10116.

Authors:

Y.Matsuzaki,F.K.Sano,H.S.Oshima,H.Akasaka,K.Kobayashi,T.Tanaka, Y.Itoh,W.Shihoya,Y.Kise,T.Kusakizako,O.Nureki

Key ref:

Y.Matsuzaki et al. Structural insights into ligand recognition and g pro preferences across histamine receptors. To be published, . PubMed id: 40579541

Date:

30-Jan-25 Release date: 11-Jun-25

Protein chain

P63092  (GNAS2_HUMAN) -  Guanine nucleotide-binding protein G(s) subunit alpha isoforms short from Homo sapiens

Seq: 394 a.a.

Struc: 231 a.a.*

Protein chain

P54311  (GBB1_RAT) -  Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 from Rattus norvegicus

Seq: 340 a.a.

Struc: 340 a.a.*

Protein chain

P63212  (GBG2_BOVIN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 from Bos taurus

Seq: 71 a.a.

Struc: 52 a.a.

Protein chain

No UniProt id for this chain

Struc: 128 a.a.

Protein chain

P35367  (HRH1_HUMAN) -  Histamine H1 receptor from Homo sapiens

Seq: 487 a.a.

Struc: 267 a.a.

Protein chain

W8GG88  (W8GG88_9HEPC) -  Genome polyprotein from Hepacivirus hominis

Seq: 3247 a.a.

Struc: 267 a.a.*

* PDB and UniProt seqs differ at 283 residue positions (black crosses)

Enzyme reactions

• Enzyme class 2: Chain A: E.C.3.6.5.- - ?????
• Enzyme class 3: Chain R: E.C.3.4.21.98 - hepacivirin.
• Reaction: Hydrolysis of four peptide bonds in the viral precursor polyprotein, commonly with Asp or Glu in the P6 position, Cys or Thr in P1 and Ser or Ala in P1'.
• Enzyme class 4: Chain R: E.C.3.6.1.15 - nucleoside-triphosphate phosphatase.
• Reaction: a ribonucleoside 5'-triphosphate + H2O = a ribonucleoside 5'-diphosphate + phosphate + H⁺
• Enzyme class 5: Chain R: E.C.3.6.4.13 - Rna helicase.
• Reaction: ATP + H2O = ADP + phosphate + H⁺

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site