PDBsum entry 9k1d

Membrane protein

PDB id: 9k1d

Contents

Protein chains

266 a.a.

219 a.a.

328 a.a.

45 a.a.

Ligands

BUA

CLR ×5

PDB id:

9k1d

Name:

Membrane protein

Title:

Cryo-em structure of the butyrate bound ffa2-gi complex

Structure:

Free fatty acid receptor 2. Chain: r. Synonym: g-protein coupled receptor 43. Engineered: yes. Guanine nucleotide-binding protein g(i) subunit alpha-1. Chain: a. Synonym: adenylate cyclase-inhibiting g alpha protein. Engineered: yes. Mutation: yes.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: ffar2, ffa2, gpcr43, gpr43. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Gene: gnai1. Gene: gnb1. Gene: gng2.

Authors:

S.Han,B.Wu,Q.Zhao

Key ref:

Y.Ke et al. (2024). Structural insights into endogenous ligand selectivit activation mechanisms of ffar1 and ffar2.. Cell rep, 43, 15024. PubMed id: 39616615

Date:

16-Oct-24 Release date: 11-Dec-24

Protein chain

O15552  (FFAR2_HUMAN) -  Free fatty acid receptor 2 from Homo sapiens

Seq: 330 a.a.

Struc: 266 a.a.

Protein chain

P63096  (GNAI1_HUMAN) -  Guanine nucleotide-binding protein G(i) subunit alpha-1 from Homo sapiens

Seq: 354 a.a.

Struc: 219 a.a.*

Protein chain

P62873  (GBB1_HUMAN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 from Homo sapiens

Seq: 340 a.a.

Struc: 328 a.a.

Protein chain

P59768  (GBG2_HUMAN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 from Homo sapiens

Seq: 71 a.a.

Struc: 45 a.a.

* PDB and UniProt seqs differ at 5 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chain A: E.C.3.6.5.- - ?????