PDBsum entry 8vap

Replication

PDB id: 8vap

Contents

Protein chains

333 a.a.

360 a.a.

334 a.a.

369 a.a.

248 a.a.

Ligands

ADP-BEF ×2

ADP

BEF

Metals

_ZN ×4

_MG ×3

PDB id:

8vap

Name:

Replication

Title:

Structure of the e. Coli clamp loader bound to the beta clamp in a fully-open conformation

Structure:

DNA polymerase iii subunit delta. Chain: a. Engineered: yes. DNA polymerase iii subunit tau. Chain: b, c, d. Synonym: DNA polymerase iii subunit gamma. Engineered: yes. DNA polymerase iii subunit delta'. Chain: e.

Source:

Escherichia coli. Organism_taxid: 562. Gene: hola. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Gene: dnax, dnaz, dnazx, b0470, jw0459. Gene: holb. Gene: dnan. Expression_system_taxid: 469008

Authors:

J.T.Landeck,B.A.Kelch

Key ref:

J.T.Landeck et al. Differences between bacteria and eukaryotes in clamp mechanism, A conserved process underlying DNA replica. J biol chem, . PubMed id: 38490435

Date:

11-Dec-23 Release date: 27-Mar-24

Protein chain

P28630  (HOLA_ECOLI) -  DNA polymerase III subunit delta from Escherichia coli (strain K12)

Seq: 343 a.a.

Struc: 333 a.a.

Protein chains

P06710  (DPO3X_ECOLI) -  DNA polymerase III subunit tau from Escherichia coli (strain K12)

Seq: 643 a.a.

Struc: 360 a.a.

Protein chain

P28631  (HOLB_ECOLI) -  DNA polymerase III subunit delta' from Escherichia coli (strain K12)

Seq: 334 a.a.

Struc: 334 a.a.

Protein chain

P0A988  (DPO3B_ECOLI) -  Beta sliding clamp from Escherichia coli (strain K12)

Seq: 366 a.a.

Struc: 369 a.a.

Protein chain

P0A988  (DPO3B_ECOLI) -  Beta sliding clamp from Escherichia coli (strain K12)

Seq: 366 a.a.

Struc: 248 a.a.

Enzyme reactions

• Enzyme class 1: Chains A, B, C, D, E: E.C.2.7.7.7 - DNA-directed Dna polymerase.
• Reaction: DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate
• Enzyme class 2: Chains F, G: E.C.?

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site