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PDBsum entry 8ff6

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protein ligands metals Protein-protein interface(s) links
Oxidoreductase PDB id
8ff6

 

 

 

 

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Contents
Protein chains
(+ 0 more) 250 a.a.
Ligands
HEM ×6
Metals
_NA ×6
Waters ×281
PDB id:
8ff6
Name: Oxidoreductase
Title: Cytosolic ascorbate peroxidase mutant from panicum virgatum
Structure: Cytosolic ascorbate peroxidase. Chain: a, b, c, d, e, f. Engineered: yes. Mutation: yes
Source: Panicum virgatum. Switchgrass. Organism_taxid: 38727. Gene: pvap13_9kg480900. Expressed in: escherichia coli. Expression_system_taxid: 562
Resolution:
2.49Å     R-factor:   0.190     R-free:   0.262
Authors: B.Zhang,C.Kang
Key ref: B.Zhang et al. (2023). Activity of cytosolic ascorbate peroxidase (apx) from panicum virgatum against ascorbate and phenylpropanoi. Int j mol sci, 24, . PubMed id: 36675291
Date:
07-Dec-22     Release date:   01-Feb-23    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
A0A8T0NWI5  (A0A8T0NWI5_PANVG) -  L-ascorbate peroxidase from Panicum virgatum
Seq:
Struc:
250 a.a.
250 a.a.*
Key:    PfamA domain  Secondary structure
* PDB and UniProt seqs differ at 5 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class: E.C.1.11.1.11  - L-ascorbate peroxidase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: L-ascorbate + H2O2 = L-dehydroascorbate + 2 H2O
L-ascorbate
+ H2O2
= L-dehydroascorbate
+ 2 × H2O
      Cofactor: Heme
Heme
Bound ligand (Het Group name = HEM) matches with 95.45% similarity
Molecule diagrams generated from .mol files obtained from the KEGG ftp site

 

 

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