PDBsum entry 8xfs

Membrane protein

PDB id: 8xfs

Contents

Protein chains

736 a.a.

185 a.a.

106 a.a.

101 a.a.

PDB id:

8xfs

Name:

Membrane protein

Title:

Lgr4-rspo2-znrf3 ring domain (1:2:2)

Structure:

Leucine-rich repeat-containing g-protein coupled receptor 4. Chain: a. Synonym: g-protein coupled receptor 48. Engineered: yes. E3 ubiquitin-protein ligase znrf3. Chain: c, e. Synonym: ring finger protein 203,ring-type e3 ubiquitin transferase znrf3,zinc/ring finger protein 3.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: lgr4, gpr48. Expressed in: homo sapiens. Expression_system_taxid: 9606. Gene: znrf3, kiaa1133, rnf203. Camelus bactrianus. Organism_taxid: 9837.

Authors:

W.Lu,G.Yong

Key ref:

W.Lu Lgr4-Rspo2-Znrf3 ring domain (1:2:2). To be published, . PubMed id: 39753551

Date:

14-Dec-23 Release date: 04-Dec-24

Protein chain

Q9BXB1  (LGR4_HUMAN) -  Leucine-rich repeat-containing G-protein coupled receptor 4 from Homo sapiens

Seq: 951 a.a.

Struc: 736 a.a.*

Protein chains

Q9ULT6  (ZNRF3_HUMAN) -  E3 ubiquitin-protein ligase ZNRF3 from Homo sapiens

Seq: 936 a.a.

Struc: 185 a.a.

Protein chain

No UniProt id for this chain

Struc: 106 a.a.

Protein chains

Q6UXX9  (RSPO2_HUMAN) -  R-spondin-2 from Homo sapiens

Seq: 243 a.a.

Struc: 101 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chains C, E: E.C.2.3.2.27 - RING-type E3 ubiquitin transferase.
• Reaction: S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N₆- ubiquitinyl-[acceptor protein]-L-lysine