PDBsum entry 8xfp

Membrane protein

PDB id: 8xfp

Contents

Protein chains

734 a.a.

114 a.a.

181 a.a.

100 a.a.

152 a.a.

PDB id:

8xfp

Name:

Membrane protein

Title:

The pentamera complex of lgr4-rspo2-znrf3(delta ring)

Structure:

Leucine-rich repeat-containing g-protein coupled receptor 4. Chain: a. Synonym: g-protein coupled receptor 48. Engineered: yes. Nanobody nb52. Chain: e. Engineered: yes. E3 ubiquitin-protein ligase znrf3.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: lgr4, gpr48. Expressed in: homo sapiens. Expression_system_taxid: 9606. Camelus bactrianus. Organism_taxid: 9837. Expressed in: escherichia coli.

Authors:

Y.Geng,L.Wang

Key ref:

Y.Geng The pentamera complex of lgr4-Rspo2-Znrf3(delta ring). To be published, . PubMed id: 39753551

Date:

14-Dec-23 Release date: 04-Dec-24

Protein chain

Q9BXB1  (LGR4_HUMAN) -  Leucine-rich repeat-containing G-protein coupled receptor 4 from Homo sapiens

Seq: 951 a.a.

Struc: 734 a.a.

Protein chain

No UniProt id for this chain

Struc: 114 a.a.

Protein chain

Q9ULT6  (ZNRF3_HUMAN) -  E3 ubiquitin-protein ligase ZNRF3 from Homo sapiens

Seq: 936 a.a.

Struc: 181 a.a.

Protein chains

Q6UXX9  (RSPO2_HUMAN) -  R-spondin-2 from Homo sapiens

Seq: 243 a.a.

Struc: 100 a.a.*

Protein chain

Q9ULT6  (ZNRF3_HUMAN) -  E3 ubiquitin-protein ligase ZNRF3 from Homo sapiens

Seq: 936 a.a.

Struc: 152 a.a.

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: Chains C, H: E.C.2.3.2.27 - RING-type E3 ubiquitin transferase.
• Reaction: S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N₆- ubiquitinyl-[acceptor protein]-L-lysine