PDBsum entry 8thl

Signaling protein

PDB id: 8thl

Contents

Protein chains

212 a.a.

338 a.a.

54 a.a.

232 a.a.

244 a.a.

Ligands

ALE

PDB id:

8thl

Name:

Signaling protein

Title:

Cryo-em structure of epinephrine-bound alpha-1a-adrenergic receptor in complex with heterotrimeric gq-protein

Structure:

Guanine nucleotide-binding protein g(i) subunit alpha-2, guanine nucleotide-binding protein g(s) subunit alpha isoforms short, guanine nucleotide-binding protein g(q) subunit alpha. Chain: a. Synonym: adenylate cyclase-inhibiting g alpha protein,adenylate cyclase-stimulating g alpha protein,guanine nucleotide-binding protein alpha-q. Engineered: yes. Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: gnai2, gnai2b, gnas, gnas1, gsp, gnaq, gaq. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Gene: gnb1. Gene: gng2. Enterobacteria phage t4, homo sapiens.

Authors:

M.Su,X.Y.Huang

Key ref:

M.Su et al. (2023). Structural basis of agonist specificity of alpha 1a-Adrenergic receptor. Nat commun, 14, 4819. PubMed id: 37563160

Date:

17-Jul-23 Release date: 16-Aug-23

Protein chain

P04899  (GNAI2_HUMAN) -  Guanine nucleotide-binding protein G(i) subunit alpha-2 from Homo sapiens

Seq: 355 a.a.

Struc: 212 a.a.*

Protein chain

P50148  (GNAQ_HUMAN) -  Guanine nucleotide-binding protein G(q) subunit alpha from Homo sapiens

Seq: 359 a.a.

Struc: 212 a.a.*

Protein chain

P63092  (GNAS2_HUMAN) -  Guanine nucleotide-binding protein G(s) subunit alpha isoforms short from Homo sapiens

Seq: 394 a.a.

Struc: 212 a.a.*

Protein chain

P62873  (GBB1_HUMAN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 from Homo sapiens

Seq: 340 a.a.

Struc: 338 a.a.

Protein chain

P59768  (GBG2_HUMAN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 from Homo sapiens

Seq: 71 a.a.

Struc: 54 a.a.

Protein chain

No UniProt id for this chain

Struc: 232 a.a.

Protein chain

P00720  (ENLYS_BPT4) -  Endolysin from Enterobacteria phage T4

Seq: 164 a.a.

Struc: 244 a.a.*

Protein chain

P35348  (ADA1A_HUMAN) -  Alpha-1A adrenergic receptor from Homo sapiens

Seq: 466 a.a.

Struc: 244 a.a.

* PDB and UniProt seqs differ at 474 residue positions (black crosses)

Enzyme reactions

• Enzyme class 1: Chain A: E.C.3.6.5.- - ?????
• Enzyme class 2: Chain R: E.C.3.2.1.17 - lysozyme.
• Reaction: Hydrolysis of the 1,4-beta-linkages between N-acetyl-D-glucosamine and N-acetylmuramic acid in peptidoglycan heteropolymers of the prokaryotes cell walls.

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.