PDBsum entry 8ihh

Signaling protein

PDB id: 8ihh

Contents

Protein chains

292 a.a.

218 a.a.

339 a.a.

56 a.a.

226 a.a.

Ligands

NAG

P8A

PDB id:

8ihh

Name:

Signaling protein

Title:

Cryo-em structure of hca2-gi complex with luf6283

Structure:

Soluble cytochrome b562,hydroxycarboxylic acid receptor 2. Chain: r. Synonym: cytochrome b-562,g-protein coupled receptor 109a,g-protein coupled receptor hm74a,niacin receptor 1,nicotinic acid receptor. Engineered: yes. Mutation: yes. Guanine nucleotide-binding protein g(i) subunit alpha-1. Chain: a. Synonym: adenylate cyclase-inhibiting g alpha protein.

Source:

Escherichia coli, homo sapiens. Human. Organism_taxid: 562, 9606. Gene: cybc, hcar2, gpr109a, hca2, hm74a, niacr1. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Homo sapiens. Organism_taxid: 9606. Gene: gnai1.

Authors:

S.Suzuki,K.Nishikawa,H.Suzuki,Y.Fujiyoshi

Key ref:

S.Suzuki et al. Structural basis of signaling mechanisms of hydroxycarboxylic acid receptors through ligand bindi. To be published, . PubMed id: 37736747

Date:

22-Feb-23 Release date: 30-Aug-23

Protein chain

P0ABE7  (C562_ECOLX) -  Soluble cytochrome b562 from Escherichia coli

Seq: 128 a.a.

Struc: 292 a.a.*

Protein chain

Q8TDS4  (HCAR2_HUMAN) -  Hydroxycarboxylic acid receptor 2 from Homo sapiens

Seq: 363 a.a.

Struc: 292 a.a.

Protein chain

P63096  (GNAI1_HUMAN) -  Guanine nucleotide-binding protein G(i) subunit alpha-1 from Homo sapiens

Seq: 354 a.a.

Struc: 218 a.a.*

Protein chain

P62874  (GBB1_MOUSE) -  Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 from Mus musculus

Seq: 340 a.a.

Struc: 339 a.a.

Protein chain

P63213  (GBG2_MOUSE) -  Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 from Mus musculus

Seq: 71 a.a.

Struc: 56 a.a.

Protein chain

No UniProt id for this chain

Struc: 226 a.a.

* PDB and UniProt seqs differ at 117 residue positions (black crosses)