PDBsum entry 8ied

Membrane protein

PDB id: 8ied

Contents

Protein chains

269 a.a.

289 a.a.

215 a.a.

338 a.a.

230 a.a.

55 a.a.

Ligands

PC1 ×2

PDB id:

8ied

Name:

Membrane protein

Title:

Cryo-em structure of gpr156-minigo-scfv16 complex

Structure:

Probable g-protein coupled receptor 156. Chain: a, b. Engineered: yes. Guanine nucleotide-binding protein g(o) subunit alpha. Chain: c. Engineered: yes. Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1. Chain: d.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: gpr156. Expressed in: homo sapiens. Expression_system_taxid: 9606. Expression_system_cell_line: hek293. Gene: gnao1. Expressed in: spodoptera frugiperda.

Authors:

J.Shin,J.Park,Y.Cho

Key ref:

J.Shin et al. Constitutive activation mechanism of a class c gpcr.. Nat struct mol biol, . PubMed id: 38332368

Date:

15-Feb-23 Release date: 14-Feb-24

Protein chain

Q8NFN8  (GP156_HUMAN) -  Probable G-protein coupled receptor 156 from Homo sapiens

Seq: 814 a.a.

Struc: 269 a.a.

Protein chain

Q8NFN8  (GP156_HUMAN) -  Probable G-protein coupled receptor 156 from Homo sapiens

Seq: 814 a.a.

Struc: 289 a.a.

Protein chain

P09471  (GNAO_HUMAN) -  Guanine nucleotide-binding protein G(o) subunit alpha from Homo sapiens

Seq: 354 a.a.

Struc: 215 a.a.*

Protein chain

P62873  (GBB1_HUMAN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 from Homo sapiens

Seq: 340 a.a.

Struc: 338 a.a.

Protein chain

No UniProt id for this chain

Struc: 230 a.a.

Protein chain

P59768  (GBG2_HUMAN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 from Homo sapiens

Seq: 71 a.a.

Struc: 55 a.a.

* PDB and UniProt seqs differ at 10 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chains A, B, C, D, Y: E.C.?