PDBsum entry 8fyt

Signaling protein

PDB id: 8fyt

Contents

Protein chains

286 a.a.

235 a.a.

336 a.a.

57 a.a.

Ligands

7LD

J40

Y01 ×2

PDB id:

8fyt

Name:

Signaling protein

Title:

Lsd-bound serotonin 1a (5-ht1a) receptor-gi1 protein complex

Structure:

Soluble cytochrome b562,5-hydroxytryptamine receptor 1a,5- hydroxytryptamine receptor 1a. Chain: r. Synonym: 5-ht-1a,5-ht1a,g-21,serotonin receptor 1a. Engineered: yes. Mutation: yes. Other_details: n-terminal ha signal sequence, flag-tag, his-tag, tev cleavage site followed by soluble cytochrome b562 (e. Coli) and truncated 5-hydroxytryptamine receptor 1a (homo sapiens) with a point

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: cybc, htr1a, adrb2rl1, adrbrl1. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Gene: gnai1. Gene: gnb1. Gene: gng2.

Authors:

A.L.Warren,G.Zilberg,M.J.Capper,D.Wacker

Key ref:

A.L.Warren et al. Structural pharmacology and therapeutic potential of 5-Methoxytryptamines. Nature, . PubMed id: 38720072

Date:

26-Jan-23 Release date: 15-May-24

Protein chain

P08908  (5HT1A_HUMAN) -  5-hydroxytryptamine receptor 1A from Homo sapiens

Seq: 422 a.a.

Struc: 286 a.a.*

Protein chain

P0ABE7  (C562_ECOLX) -  Soluble cytochrome b562 from Escherichia coli

Seq: 128 a.a.

Struc: 286 a.a.*

Protein chain

P63096  (GNAI1_HUMAN) -  Guanine nucleotide-binding protein G(i) subunit alpha-1 from Homo sapiens

Seq: 354 a.a.

Struc: 235 a.a.

Protein chain

P62873  (GBB1_HUMAN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 from Homo sapiens

Seq: 340 a.a.

Struc: 336 a.a.

Protein chain

P59768  (GBG2_HUMAN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 from Homo sapiens

Seq: 71 a.a.

Struc: 57 a.a.

* PDB and UniProt seqs differ at 112 residue positions (black crosses)